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Sample GSM720950 Query DataSets for GSM720950
Status Public on Oct 01, 2011
Title p62_empty_vector
Sample type RNA
 
Source name Human Bone marrow
Organism Homo sapiens
Characteristics tissue origin: normal human bone marrow
cell type: mononuclear cells
Treatment protocol Stimulated with M-CSF and RANKL for 48 h.
Growth protocol Human bone marrow mononuclear cells transduced with p62-EV, p62WT, p62P392L, retroviral expression vectors.
Extracted molecule total RNA
Extraction protocol Total RNA isolated from above treated pre-osteoclast cells.
Label Alexa-555
Label protocol First and second strand cDNA was prepared from the total RNA samples. cRNA target was prepared from the DNA template and verified on the Bioanalyzer. cRNA was fragmented to uniform size and verified on the Bioanalyzer.
 
Hybridization protocol 1 µg of purified cRNA was fragmented to uniform size and applied to Agilent Whole Human Genome microarrays (Agilent Technologies, Santa Clara, CA) in hybridization buffer. Arrays were hybridized at 65° C for 17 hrs. in a rotating incubator, washed at 37° C for 1 min, and stained with Streptavidin-Alexa555.
Scan protocol Rinsed and dried arrays were scanned with an Agilent G2565 Microarray Scanner (Agilent Technologies, Santa Clara, CA) at 5 µm resolution.
Description EV
Data processing Agilent Feature Extraction software was used to process the scanned images from arrays (gridding and feature intensity extraction) and the data generated for each probe on the array was analyzed with GeneSpring GX v7.3.1 software (Agilent Technologies, Santa Clara, CA).
 
Submission date May 06, 2011
Last update date Oct 01, 2011
Contact name Michael Falduto
E-mail(s) mfalduto@genusbiosystems.com
Phone 847-291-9602
Organization name GenUs BioSystems, Inc.
Street address 1808 Janke, Unit M
City Northbrook
State/province IL
ZIP/Postal code 60062
Country USA
 
Platform ID GPL13497
Series (1)
GSE29107 Microarray profile of p62P392L-regulated gene expression in RANKL and M-CSF stimulated normal human bone marrow derived non-adherent cells

Data table header descriptions
ID_REF
VALUE Intensity values are normalized to the 75th percentile intensity of each array.

Data table
ID_REF VALUE
A_23_P100001 0.370
A_23_P100022 0.073
A_23_P100056 0.018
A_23_P100074 0.739
A_23_P100127 0.054
A_23_P100141 0.472
A_23_P100189 0.037
A_23_P100196 1.614
A_23_P100203 1.544
A_23_P100220 0.477
A_23_P100240 0.016
A_23_P10025 0.554
A_23_P100292 8.667
A_23_P100315 4.563
A_23_P100326 26.539
A_23_P100344 0.813
A_23_P100355 17.791
A_23_P100386 0.021
A_23_P100392 2.093
A_23_P100420 0.540

Total number of rows: 34127

Table truncated, full table size 644 Kbytes.




Supplementary file Size Download File type/resource
GSM720950_P62-EV.txt.gz 9.0 Mb (ftp)(http) TXT
Processed data included within Sample table

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