|
| Status |
Public on Oct 01, 2011 |
| Title |
p62_P392L |
| Sample type |
RNA |
| |
|
| Source name |
Human Bone marrow
|
| Organism |
Homo sapiens |
| Characteristics |
tissue origin: normal human bone marrow
cell type: mononuclear cells
|
| Treatment protocol |
Stimulated with M-CSF and RANKL for 48 h.
|
| Growth protocol |
Human bone marrow mononuclear cells transduced with p62-EV, p62WT, p62P392L, retroviral expression vectors.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA isolated from above treated pre-osteoclast cells.
|
| Label |
Alexa-555
|
| Label protocol |
First and second strand cDNA was prepared from the total RNA samples. cRNA target was prepared from the DNA template and verified on the Bioanalyzer. cRNA was fragmented to uniform size and verified on the Bioanalyzer.
|
| |
|
| Hybridization protocol |
1 µg of purified cRNA was fragmented to uniform size and applied to Agilent Whole Human Genome microarrays (Agilent Technologies, Santa Clara, CA) in hybridization buffer. Arrays were hybridized at 65° C for 17 hrs. in a rotating incubator, washed at 37° C for 1 min, and stained with Streptavidin-Alexa555.
|
| Scan protocol |
Rinsed and dried arrays were scanned with an Agilent G2565 Microarray Scanner (Agilent Technologies, Santa Clara, CA) at 5 µm resolution.
|
| Description |
P62-Ex8
|
| Data processing |
Agilent Feature Extraction software was used to process the scanned images from arrays (gridding and feature intensity extraction) and the data generated for each probe on the array was analyzed with GeneSpring GX v7.3.1 software (Agilent Technologies, Santa Clara, CA).
|
| |
|
| Submission date |
May 06, 2011 |
| Last update date |
Oct 01, 2011 |
| Contact name |
Michael Falduto |
| E-mail(s) |
mfalduto@genusbiosystems.com
|
| Phone |
847-291-9602
|
| Organization name |
GenUs BioSystems, Inc.
|
| Street address |
1808 Janke, Unit M
|
| City |
Northbrook |
| State/province |
IL |
| ZIP/Postal code |
60062 |
| Country |
USA |
| |
|
| Platform ID |
GPL13497 |
| Series (1) |
| GSE29107 |
Microarray profile of p62P392L-regulated gene expression in RANKL and M-CSF stimulated normal human bone marrow derived non-adherent cells |
|
