|
| Status |
Public on May 20, 2011 |
| Title |
TRAMP_serum_advancedDiseased_rep3 |
| Sample type |
RNA |
| |
|
| Source name |
Serum from TRAMP mice with advanced disease
|
| Organism |
Mus musculus |
| Characteristics |
tissue: serum
strain: C57BL/6 TRAMP x FVB
genotype/variation: TRAMP
age: mixed
|
| Treatment protocol |
NA
|
| Growth protocol |
NA
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted twice with phenol:chloroform and precipitated using ethanol.
|
| Label |
biotin
|
| Label protocol |
Approximately 800ng of RNA was labelled using the FlashTag HSR Biotin RNA Labelling Kit (Genisphere), according to the manufacturer's instructions.
|
| |
|
| Hybridization protocol |
cRNA were hybridized for 16 hr at 48C on GeneChip miRNA Arrays and then washed and stained with a Steptavidin-PE solution prior to imaging.
|
| Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
|
| Description |
miRNA data from TRAMP serum
|
| Data processing |
Data were background corrected and statistically analysed using Partek Genomics Suite (Partek Inc.) using the subset of mouse probes on the array. Raw data was processed using RMA background correction and Partek’s own GC content correction with mean probe summarization.
|
| |
|
| Submission date |
May 16, 2011 |
| Last update date |
May 20, 2011 |
| Contact name |
Luke Selth |
| E-mail(s) |
luke.selth@flinders.edu.au
|
| Organization name |
Flinders University
|
| Department |
College of Medicine and Public Health
|
| Street address |
Flinders Drive
|
| City |
Bedford Park |
| State/province |
SA |
| ZIP/Postal code |
5042 |
| Country |
Australia |
| |
|
| Platform ID |
GPL8786 |
| Series (1) |
| GSE29314 |
Identification of serum microRNAs associated with prostate cancer in a mouse model of disease |
|
