|
| Status |
Public on Dec 20, 2011 |
| Title |
Cell transfected with Pax8 siRNA rep2 |
| Sample type |
RNA |
| |
|
| Source name |
FRTL-5 differentiated thyroid cells silenced for Pax8
|
| Organism |
Rattus norvegicus |
| Characteristics |
knockdown: silenced for Pax8
cell line: FRTL-5
|
| Treatment protocol |
FRTL-5 cells were transfected in triplicate with 100 nM Pax8 siGENOME siRNA (siPax8 samples) or siGENOME Non-Targeting #3 (DHARMACON) as scramble (ntPax8 control samples) using DharmaFECT 1 transfection reagent, following the manufacturer’s protocol.
|
| Growth protocol |
FRTL-5 cells were grown in Coon’s modified F-12 medium (Euroclone) supplemented with 5% calf serum and a six-hormone mixture
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted 72 hours after transfection using TRIzol reagent (Invitrogen) and treated with RNase-free DNase I (Applied Biosystem)
|
| Label |
biotin
|
| Label protocol |
According to Affymetrix protocol described in GeneChip® Expression Analysis Technical Manual at http://www.affymetrix.com
|
| |
|
| Hybridization protocol |
According to Affymetrix protocol described in GeneChip® Expression Analysis Technical Manual at http://www.affymetrix.com
|
| Scan protocol |
According to Affymetrix protocol described in GeneChip® Expression Analysis Technical Manual at http://www.affymetrix.com
|
| Description |
RNA from cells 72hrs after transfection
|
| Data processing |
Gene-level signals were generated from CEL files by GeneSpring v.11.5 software (Agilent Technologies Inc.), using the Probe Logarithmic Intensity Error (PLIER16) summarization algorithm
RaGene-1_0-st-v1.r4.pgf
|
| |
|
| Submission date |
May 17, 2011 |
| Last update date |
Dec 20, 2011 |
| Contact name |
Lucio Nitsch |
| E-mail(s) |
nitsch@unina.it
|
| Phone |
+39-0817463621
|
| Fax |
+39-0817701016
|
| Organization name |
Unversity of Naples Federico II
|
| Department |
Biologia e Patologia Cellulare e Molecolare
|
| Street address |
Via Pansini 5
|
| City |
Napoli |
| ZIP/Postal code |
80131 |
| Country |
Italy |
| |
|
| Platform ID |
GPL6247 |
| Series (1) |
| GSE29341 |
Gene expression profile changes upon knock-down of Pax8 |
|
