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Sample GSM726061 Query DataSets for GSM726061
Status Public on Feb 16, 2012
Title DMSO+BMP-2 rep2
Sample type RNA
 
Source name C2C12 cells
Organism Mus musculus
Characteristics cell type: myoblast
cell line: C2C12 cells
treatment: DMSO+BMP-2
Treatment protocol C2C12 cells were serum-starved for 4 h, after 2 h 40 µM dynasore or 0.05 % DMSO was added to the medium. Cells were stimulated for 6 h with 30 nM BMP-2 including dynasore or DMSO.
Growth protocol C2C12 cells were cultured according to standard protocols.
Extracted molecule total RNA
Extraction protocol Total RNA was isolated using NucleoSpin RNA II Isolation Kit (Machery-Nagel) followed by quality assessment using both spectrophotometer (NanoDrop Technologies) and agarose gel electrophoresis.
Label biotin
Label protocol Biotin-labelled cRNA was generated, employing a linear amplification kit (Ambion).
 
Hybridization protocol Standard Illumina hybridization protocol
Scan protocol Standard Illumina scanning protocol
Description replicate 2
Data processing Rank invariant normalization was performed using the algorithm from BeadStudio 3.0 software (Illumina)
 
Submission date May 18, 2011
Last update date Feb 16, 2012
Contact name Raghu Bhushan
E-mail(s) raghubhushanin@yahoo.co.in
Phone 00493083852945
Fax 00493083852936
Organization name Freie Universität
Department Institut für Chemie und Biochemie
Lab Knaus lab
Street address Thielallee 63
City Berlin
State/province Berlin
ZIP/Postal code 14195
Country Germany
 
Platform ID GPL6885
Series (1)
GSE29373 Spatial segregation of BMP/Smad signaling affects osteoblast differentiation in C2C12 cells

Data table header descriptions
ID_REF
VALUE Rank invariant normalized
Detection Pval

Data table
ID_REF VALUE Detection Pval
ILMN_2721178 359.0966 0.08447183
ILMN_3092673 3472.447 0.000314068
ILMN_2816356 241.2291 0.4887218
ILMN_2808939 706.6377 0
ILMN_2634564 1013.839 0
ILMN_2737647 246.4992 0.3233083
ILMN_2734484 784.519 0
ILMN_2699078 347.9055 0.1712371
ILMN_1213681 264.3739 0.05137845
ILMN_2735415 238.1444 0.7192197
ILMN_2891688 551.8729 0
ILMN_2637698 938.8386 0
ILMN_2674228 269.5375 0.02882206
ILMN_2601546 276.2432 0.01503759
ILMN_1230831 250.1433 0.2243108
ILMN_2848071 248.2809 0.199446
ILMN_2658619 1692.692 0
ILMN_2682917 249.7217 0.1458541
ILMN_2973337 230.2685 0.8433584
ILMN_1226653 561.0303 0

Total number of rows: 18138

Table truncated, full table size 545 Kbytes.




Supplementary data files not provided
Processed data included within Sample table

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