|
Status |
Public on Feb 16, 2012 |
Title |
Dynasore rep2 |
Sample type |
RNA |
|
|
Source name |
C2C12 cells
|
Organism |
Mus musculus |
Characteristics |
cell type: myoblast cell line: C2C12 cells treatment: Dynasore
|
Treatment protocol |
C2C12 cells were serum-starved for 4 h, after 2 h 40 µM dynasore or 0.05 % DMSO was added to the medium. Cells were stimulated for 6 h with 30 nM BMP-2 including dynasore or DMSO.
|
Growth protocol |
C2C12 cells were cultured according to standard protocols.
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA was isolated using NucleoSpin RNA II Isolation Kit (Machery-Nagel) followed by quality assessment using both spectrophotometer (NanoDrop Technologies) and agarose gel electrophoresis.
|
Label |
biotin
|
Label protocol |
Biotin-labelled cRNA was generated, employing a linear amplification kit (Ambion).
|
|
|
Hybridization protocol |
Standard Illumina hybridization protocol
|
Scan protocol |
Standard Illumina scanning protocol
|
Description |
replicate 2
|
Data processing |
Rank invariant normalization was performed using the algorithm from BeadStudio 3.0 software (Illumina)
|
|
|
Submission date |
May 18, 2011 |
Last update date |
Feb 16, 2012 |
Contact name |
Raghu Bhushan |
E-mail(s) |
raghubhushanin@yahoo.co.in
|
Phone |
00493083852945
|
Fax |
00493083852936
|
Organization name |
Freie Universität
|
Department |
Institut für Chemie und Biochemie
|
Lab |
Knaus lab
|
Street address |
Thielallee 63
|
City |
Berlin |
State/province |
Berlin |
ZIP/Postal code |
14195 |
Country |
Germany |
|
|
Platform ID |
GPL6885 |
Series (1) |
GSE29373 |
Spatial segregation of BMP/Smad signaling affects osteoblast differentiation in C2C12 cells |
|