|
| Status |
Public on Feb 16, 2012 |
| Title |
Dynasore+BMP-2 rep2 |
| Sample type |
RNA |
| |
|
| Source name |
C2C12 cells
|
| Organism |
Mus musculus |
| Characteristics |
cell type: myoblast
cell line: C2C12 cells
treatment: Dynasore+BMP-2
|
| Treatment protocol |
C2C12 cells were serum-starved for 4 h, after 2 h 40 µM dynasore or 0.05 % DMSO was added to the medium. Cells were stimulated for 6 h with 30 nM BMP-2 including dynasore or DMSO.
|
| Growth protocol |
C2C12 cells were cultured according to standard protocols.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated using NucleoSpin RNA II Isolation Kit (Machery-Nagel) followed by quality assessment using both spectrophotometer (NanoDrop Technologies) and agarose gel electrophoresis.
|
| Label |
biotin
|
| Label protocol |
Biotin-labelled cRNA was generated, employing a linear amplification kit (Ambion).
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
replicate 2
|
| Data processing |
Rank invariant normalization was performed using the algorithm from BeadStudio 3.0 software (Illumina)
|
| |
|
| Submission date |
May 18, 2011 |
| Last update date |
Feb 16, 2012 |
| Contact name |
Raghu Bhushan |
| E-mail(s) |
raghubhushanin@yahoo.co.in
|
| Phone |
00493083852945
|
| Fax |
00493083852936
|
| Organization name |
Freie Universität
|
| Department |
Institut für Chemie und Biochemie
|
| Lab |
Knaus lab
|
| Street address |
Thielallee 63
|
| City |
Berlin |
| State/province |
Berlin |
| ZIP/Postal code |
14195 |
| Country |
Germany |
| |
|
| Platform ID |
GPL6885 |
| Series (1) |
| GSE29373 |
Spatial segregation of BMP/Smad signaling affects osteoblast differentiation in C2C12 cells |
|
