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| Status |
Public on Sep 01, 2005 |
| Title |
C2C12-puro 2, chip MOE430B |
| Sample type |
RNA |
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| Source name |
C2C12, Muscle
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| Organism |
Mus musculus |
| Characteristics |
Strain: C3H
Karyotype: Normal, diploid
Defining markers: myoD/myf5
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| Growth protocol |
Growth medium: DMEM
Media supplements: 10% FCS (heat inactivated), Penicillin/Streptomycin
Culture conditions: 37 C, 5% CO2
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| Extracted molecule |
total RNA |
| Extraction protocol |
RNA prep method: RNeasy
Cell purification method: 1. Cell line, 2. Puromycin selection
Contaminating cell type: None
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| Label |
Biotin
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| Description |
This sample was analyzed as part of the Stem Cell Genomics Project (http://www.scgp.ca:8080/StemBase/). The biological material was submitted to the Ontario Genomics Innovation Centre (http://www.ottawagenomecenter.ca/) by Dr. Michael Rudnicki (mrudnicki@ohri.ca; 501 Smyth Road) for analysis.
Stembase Experiment ID: E102
Stembase Experiment ID link: http://www.scgp.ca:8080/StemBase/?path=/browse/experiment&id=102
SCGP Sample ID: S137
SCGP Sample ID link: http://www.scgp.ca:8080/StemBase/?path=/browse/experiment&id=102#SAMPLE_102
Short description: C2C12 myoblasts infected with empty virus (puro).
Estimated purity: 100%
RNA concentration: 2.8 ug/ul
Num cells for RNA prep: >6x10e6
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| Data processing |
Calculated using the MAS5 algorithm where sc=1500, tau=0.015, alpha1=0.04, and alpla2=0.06
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| Submission date |
Aug 30, 2005 |
| Last update date |
Jan 19, 2006 |
| Organization |
Ottawa Hospital Research Institute |
| Phone |
(613) 737-8899 -73255
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| Department |
Cellular and Molecular Medicine
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| Lab |
Ottawa Bioinformatics Core Facility
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| Street address |
501 Smyth Rd.
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| City |
Ottawa |
| State/province |
ON |
| ZIP/Postal code |
K1H 8L6 |
| Country |
Canada |
| |
|
| Platform ID |
GPL340 |
| Series (1) |
| GSE3224 |
Comparison of C212 Myoblasts Infected with a Retrovirus Expressing Pax7d or an Empty Virus (puro) |
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| Data table header descriptions |
| ID_REF |
|
| VALUE |
'signal' a measure of the abundance of a transcript. Calculated using the MAS5 algorithm where sc=1500, tau=0.015, alpha1=0.04, and alpla2=0.06. |
| ABS_CALL |
the call in an absolute analysis that indicates if the transcript was present (P), absent (A), marginal (M), or no call (NC) |
| DETECTION P-VALUE |
'detection p-value', p-value that indicates the significance level of the detection call |
| Data table |
| ID_REF |
VALUE |
ABS_CALL |
DETECTION P-VALUE |
| AFFX-BioB-5_at |
21662.5 |
P |
0.000340305 |
| AFFX-BioB-M_at |
50120.1 |
P |
6.02111e-05 |
| AFFX-BioB-3_at |
19716.4 |
P |
5.16732e-05 |
| AFFX-BioC-5_at |
46624.9 |
P |
7.00668e-05 |
| AFFX-BioC-3_at |
40453.8 |
P |
5.16732e-05 |
| AFFX-BioDn-5_at |
43167.7 |
P |
4.42873e-05 |
| AFFX-BioDn-3_at |
260533 |
P |
7.00668e-05 |
| AFFX-CreX-5_at |
507618 |
P |
4.42873e-05 |
| AFFX-CreX-3_at |
738027 |
P |
4.42873e-05 |
| AFFX-DapX-5_at |
74380.2 |
P |
5.16732e-05 |
| AFFX-DapX-M_at |
153453 |
P |
6.02111e-05 |
| AFFX-DapX-3_at |
221375 |
P |
4.42873e-05 |
| AFFX-LysX-5_at |
12142.1 |
P |
4.42873e-05 |
| AFFX-LysX-M_at |
27041.6 |
P |
7.00668e-05 |
| AFFX-LysX-3_at |
49872.3 |
P |
4.42873e-05 |
| AFFX-PheX-5_at |
5809.76 |
P |
0.000445901 |
| AFFX-PheX-M_at |
5634.75 |
P |
0.002867 |
| AFFX-PheX-3_at |
15346.5 |
P |
0.00110197 |
| AFFX-ThrX-5_at |
4456.07 |
P |
0.0200219 |
| AFFX-ThrX-M_at |
3417.2 |
A |
0.0780018 |
Total number of rows: 22575
Table truncated, full table size 662 Kbytes.
| Supplementary file |
Size |
Download |
File type/resource |
| GSM72631.CEL.gz |
3.0 Mb |
(ftp)(http) |
CEL |
| GSM72631.EXP.gz |
540 b |
(ftp)(http) |
EXP |
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