NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Sample GSM72863 Query DataSets for GSM72863
Status Public on Sep 01, 2005
Title V6.5 mEB 9d-1, chip MOE430B
Sample type RNA
 
Source name V6.5 (P18), Embryonic
Organism Mus musculus
Characteristics Strain: 129SV x C57Bl/6
Biomaterial provider Contact details for sample vendor: V6.5 cell line originally obtained from Dr. R. Jaenesch's lab in Boston
Treatment protocol Assays to confirm viability: Stratagene mycoplasma PCR test
Growth protocol Growth medium: ES Cell Medium - DMEM (high glucose)
Media supplements: Hyclone FCS (heat inactivated), non-essential amino acids, Glutamax, Penicillin/Streptomycin, beta-mercaptoethanol
Culture conditions: 37 C, 5% CO2
Extracted molecule total RNA
Extraction protocol RNA prep method: RNeasy
Cell purification method: Cultured
Contaminating cell type: none
Label Biotin
 
Description This sample was analyzed as part of the Stem Cell Genomics Project (http://www.scgp.ca:8080/StemBase/). The biological material was submitted to the Ontario Genomics Innovation Centre (http://www.ottawagenomecenter.ca/) by Dr. Michael Rudnicki (mrudnicki@ohri.ca; 501 Smyth Road) for analysis.
Stembase Experiment ID: E113
Stembase Experiment ID link: http://www.scgp.ca:8080/StemBase/?path=/browse/experiment&id=113
SCGP Sample ID: S241
SCGP Sample ID link: http://www.scgp.ca:8080/StemBase/?path=/browse/experiment&id=113#SAMPLE_202
Short description: 9 days of an 11-point time course study of V6.5 embryonic stem cells and their cognate differentiated embryoid bodies. Time course: 0h, 6h, 12h, 18h, 24h, 36h, 48h, 4 days, 7days, 9 days and 15 days. All samples in triplicate. The cells are differentiating EBs from murine Embryonic Stem Cells, Mouse strain 129 crossed with C67BL/6 isolate V6.5 (P18), obtained from Dr. Josee Coulombe in Dr. M. Rudnicki's lab.
Estimated purity: 100%
RNA concentration: 2.0 ug/ul
Num cells for RNA prep: Unable to break up embryoid bodies to do a cell count.
Sample volume: 15 ul
Data processing Calculated using the MAS5 algorithm where sc=1500, tau=0.015, alpha1=0.04, and alpla2=0.06
 
Submission date Aug 30, 2005
Last update date Jan 19, 2006
Organization Ottawa Hospital Research Institute
Phone (613) 737-8899 -73255
Department Cellular and Molecular Medicine
Lab Ottawa Bioinformatics Core Facility
Street address 501 Smyth Rd.
City Ottawa
State/province ON
ZIP/Postal code K1H 8L6
Country Canada
 
Platform ID GPL340
Series (1)
GSE3231 V6.5 Embryonic Stem Cell and Embryoid Body Time Course

Data table header descriptions
ID_REF
VALUE 'signal' a measure of the abundance of a transcript. Calculated using the MAS5 algorithm where sc=1500, tau=0.015, alpha1=0.04, and alpla2=0.06.
ABS_CALL the call in an absolute analysis that indicates if the transcript was present (P), absent (A), marginal (M), or no call (NC)
DETECTION P-VALUE 'detection p-value', p-value that indicates the significance level of the detection call

Data table
ID_REF VALUE ABS_CALL DETECTION P-VALUE
AFFX-BioB-5_at 11487.3 P 0.00110197
AFFX-BioB-M_at 14637.7 P 9.4506e-05
AFFX-BioB-3_at 6922.41 P 0.000445901
AFFX-BioC-5_at 21351.5 P 8.14279e-05
AFFX-BioC-3_at 16826.9 P 5.16732e-05
AFFX-BioDn-5_at 15703.5 P 6.02111e-05
AFFX-BioDn-3_at 132046 P 8.14279e-05
AFFX-CreX-5_at 225789 P 4.42873e-05
AFFX-CreX-3_at 315890 P 4.42873e-05
AFFX-DapX-5_at 64639.6 P 4.42873e-05
AFFX-DapX-M_at 158664 P 5.16732e-05
AFFX-DapX-3_at 240479 P 4.42873e-05
AFFX-LysX-5_at 18741.6 P 4.42873e-05
AFFX-LysX-M_at 29549.2 P 4.42873e-05
AFFX-LysX-3_at 66297.7 P 4.42873e-05
AFFX-PheX-5_at 6247.42 P 8.14279e-05
AFFX-PheX-M_at 7984.43 P 0.000258358
AFFX-PheX-3_at 12338 P 0.00010954
AFFX-ThrX-5_at 3459.55 P 0.00933744
AFFX-ThrX-M_at 3620.36 P 0.0103167

Total number of rows: 22575

Table truncated, full table size 666 Kbytes.




Supplementary file Size Download File type/resource
GSM72863.CEL.gz 3.2 Mb (ftp)(http) CEL
GSM72863.EXP.gz 498 b (ftp)(http) EXP

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap