Strain: C57BL/6 Gender: Male Defining markers: Sca-1 and cKit
Treatment protocol
Assays to confirm viability: Colony forming cell (CFC) and cobblestone area forming cell (CAFC) assays as well as Trypan Blue dye exclusion to estimate the viability
This sample was analyzed as part of the Stem Cell Genomics Project (http://www.scgp.ca:8080/StemBase/). The biological material was submitted to the Ontario Genomics Innovation Centre (http://www.ottawagenomecenter.ca/) by Dr. Michael Rudnicki (mrudnicki@ohri.ca; 501 Smyth Road) for analysis. Stembase Experiment ID: E207 Stembase Experiment ID link: http://www.scgp.ca:8080/StemBase/?path=/browse/experiment&id=207 SCGP Sample ID: S294 SCGP Sample ID link: http://www.scgp.ca:8080/StemBase/?path=/browse/experiment&id=207#SAMPLE_262 Short description: This population of murine bone marrow cells is believed to be highly enriched for hematopoietic progenitor and stem cell activity. Analysis of the gene expression profile for this population might lead to identification of unique markers that are exclusively expressed by the hematopoietic stem cells. Estimated purity: > 90% RNA concentration: 10 ng/ul Num cells for RNA prep: 7.6-29 x 10^4 Sample volume: 3 ul
Data processing
Calculated using the MAS5 algorithm where sc=1500, tau=0.015, alpha1=0.04, and alpla2=0.06
