This sample was analyzed as part of the Stem Cell Genomics Project (http://www.scgp.ca:8080/StemBase/). The biological material was submitted to the Ontario Genomics Innovation Centre (http://www.ottawagenomecenter.ca/) by Dr. Michael Rudnicki (mrudnicki@ohri.ca; 501 Smyth Road) for analysis. Stembase Experiment ID: E209 Stembase Experiment ID link: http://www.scgp.ca:8080/StemBase/?path=/browse/experiment&id=209 SCGP Sample ID: S274 SCGP Sample ID link: http://www.scgp.ca:8080/StemBase/?path=/browse/experiment&id=209#SAMPLE_284 Short description: Cells were isolated from muscle (hind leg) of Balb/c mouse (male). Then cells were enriched at FACS by using Sca1 antigen. Cells were grown 4 days in NSC medium, passaged and RNA isolated. Cells were at passage 1. Analysis requested: Murinr chip MOE 430 A, B RNA concentration: small sample Num cells for RNA prep: from 2E+06 cells (FACS enrichment) Sample volume: 5uL
Data processing
Calculated using the MAS5 algorithm where sc=1500, tau=0.015, alpha1=0.04, and alpla2=0.06
