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Sample GSM72923 Query DataSets for GSM72923
Status Public on Oct 07, 2005
Title 10 T1/2 5, chip MOE430B
Sample type RNA
 
Source name 10 T1/2, embroyonic fibroblast
Organism Mus musculus
Characteristics Strain:
Growth protocol Growth medium: DMEM
Media supplements: 10% FBS
Culture conditions: harvested at 80% confluency
Extracted molecule total RNA
Extraction protocol RNA prep method: RNeasy
Label Biotin
 
Description This sample was analyzed as part of the Stem Cell Genomics Project (http://www.scgp.ca:8080/StemBase/). The biological material was submitted to the Ontario Genomics Innovation Centre (http://www.ottawagenomecenter.ca/) by Dr. Michael Rudnicki (mrudnicki@ohri.ca; 501 Smyth Road) for analysis.
Stembase Experiment ID: E211
Stembase Experiment ID link: http://www.scgp.ca:8080/StemBase/?path=/browse/experiment&id=211
SCGP Sample ID: S286
SCGP Sample ID link: http://www.scgp.ca:8080/StemBase/?path=/browse/experiment&id=211#SAMPLE_295
Short description: 10 T1/2 shares a set of genes with stem cells. 10 T1/2 microarray data will be used to subtract these common genes from gene profiles of other stem cell samples. Three best samples to be used for microarray experiment.
Analysis requested: MOE 430A and B
RNA concentration: 100ng/uL
Sample volume: 5uL
Data processing Calculated using the MAS5 algorithm where sc=1500, tau=0.015, alpha1=0.04, and alpla2=0.06
 
Submission date Aug 30, 2005
Last update date Jan 19, 2006
Organization Ottawa Hospital Research Institute
Phone (613) 737-8899 -73255
Department Cellular and Molecular Medicine
Lab Ottawa Bioinformatics Core Facility
Street address 501 Smyth Rd.
City Ottawa
State/province ON
ZIP/Postal code K1H 8L6
Country Canada
 
Platform ID GPL340
Series (1)
GSE3236 10 T1/2 Embryonic Fibroblasts

Data table header descriptions
ID_REF
VALUE 'signal' a measure of the abundance of a transcript. Calculated using the MAS5 algorithm where sc=1500, tau=0.015, alpha1=0.04, and alpla2=0.06.
ABS_CALL the call in an absolute analysis that indicates if the transcript was present (P), absent (A), marginal (M), or no call (NC)
DETECTION P-VALUE 'detection p-value', p-value that indicates the significance level of the detection call

Data table
ID_REF VALUE ABS_CALL DETECTION P-VALUE
AFFX-BioB-5_at 10427 P 0.0020226
AFFX-BioB-M_at 14598.4 P 5.16732e-05
AFFX-BioB-3_at 7391.79 P 5.16732e-05
AFFX-BioC-5_at 25034.3 P 0.000169227
AFFX-BioC-3_at 14697.7 P 4.42873e-05
AFFX-BioDn-5_at 15607.5 P 9.4506e-05
AFFX-BioDn-3_at 92014.1 P 7.00668e-05
AFFX-CreX-5_at 200832 P 4.42873e-05
AFFX-CreX-3_at 289309 P 4.42873e-05
AFFX-DapX-5_at 100172 P 4.42873e-05
AFFX-DapX-M_at 502828 P 4.42873e-05
AFFX-DapX-3_at 589183 P 4.42873e-05
AFFX-LysX-5_at 77875.8 P 4.42873e-05
AFFX-LysX-M_at 434243 P 4.42873e-05
AFFX-LysX-3_at 481450 P 4.42873e-05
AFFX-PheX-5_at 25580.2 P 5.16732e-05
AFFX-PheX-M_at 80712.6 P 4.42873e-05
AFFX-PheX-3_at 467303 P 4.42873e-05
AFFX-ThrX-5_at 3499.49 P 0.0032123
AFFX-ThrX-M_at 11989.7 P 0.000258358

Total number of rows: 22575

Table truncated, full table size 665 Kbytes.




Supplementary file Size Download File type/resource
GSM72923.CEL.gz 3.1 Mb (ftp)(http) CEL
GSM72923.EXP.gz 508 b (ftp)(http) EXP

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