Growth medium: DMEM Media supplements: 10% FBS Culture conditions: harvested at 80% confluency
Extracted molecule
total RNA
Extraction protocol
RNA prep method: RNeasy
Label
Biotin
Description
This sample was analyzed as part of the Stem Cell Genomics Project (http://www.scgp.ca:8080/StemBase/). The biological material was submitted to the Ontario Genomics Innovation Centre (http://www.ottawagenomecenter.ca/) by Dr. Michael Rudnicki (mrudnicki@ohri.ca; 501 Smyth Road) for analysis. Stembase Experiment ID: E211 Stembase Experiment ID link: http://www.scgp.ca:8080/StemBase/?path=/browse/experiment&id=211 SCGP Sample ID: S286 SCGP Sample ID link: http://www.scgp.ca:8080/StemBase/?path=/browse/experiment&id=211#SAMPLE_295 Short description: 10 T1/2 shares a set of genes with stem cells. 10 T1/2 microarray data will be used to subtract these common genes from gene profiles of other stem cell samples. Three best samples to be used for microarray experiment. Analysis requested: MOE 430A and B RNA concentration: 100ng/uL Sample volume: 5uL
Data processing
Calculated using the MAS5 algorithm where sc=1500, tau=0.015, alpha1=0.04, and alpla2=0.06