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| Status |
Public on Sep 01, 2005 |
| Title |
CPGSE#6, chip MOE430B |
| Sample type |
RNA |
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| Source name |
haematopoeitic stem cells, bone marrow
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| Organism |
Mus musculus |
| Characteristics |
Strain: C57BL/6
Gender: Female
Defining markers: CD45+ScaI+
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| Extracted molecule |
total RNA |
| Extraction protocol |
RNA prep method: Qiagen
Cell purification method: Moflo cell sorter
Method for estimating purity: cell sorter reading
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| Label |
Biotin
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| Description |
This sample was analyzed as part of the Stem Cell Genomics Project (http://www.scgp.ca:8080/StemBase/). The biological material was submitted to the Ontario Genomics Innovation Centre (http://www.ottawagenomecenter.ca/) by Dr. Michael Rudnicki (mrudnicki@ohri.ca; 501 Smyth Road) for analysis.
Stembase Experiment ID: E191
Stembase Experiment ID link: http://www.scgp.ca:8080/StemBase/?path=/browse/experiment&id=191
SCGP Sample ID: S147
SCGP Sample ID link: http://www.scgp.ca:8080/StemBase/?path=/browse/experiment&id=191#SAMPLE_109
Short description: Mouse bone marrow stained with CD45-APC & Scal-PE, sorted on Moflo for +/+ cells
Analysis requested: microarray
Estimated purity: 95-98%
RNA concentration: 50ng/ul
Num cells for RNA prep: 9.4 X 10^5
Sample volume: 10ul
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| Data processing |
Calculated using the MAS5 algorithm where sc=1500, tau=0.015, alpha1=0.04, and alpla2=0.06
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| Submission date |
Aug 31, 2005 |
| Last update date |
Jan 19, 2006 |
| Organization |
Ottawa Hospital Research Institute |
| Phone |
(613) 737-8899 -73255
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| Department |
Cellular and Molecular Medicine
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| Lab |
Ottawa Bioinformatics Core Facility
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| Street address |
501 Smyth Rd.
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| City |
Ottawa |
| State/province |
ON |
| ZIP/Postal code |
K1H 8L6 |
| Country |
Canada |
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| Platform ID |
GPL340 |
| Series (1) |
| GSE3241 |
Sca1-CD45 sorted bone marrow |
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| Data table header descriptions |
| ID_REF |
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| VALUE |
'signal' a measure of the abundance of a transcript. Calculated using the MAS5 algorithm where sc=1500, tau=0.015, alpha1=0.04, and alpla2=0.06. |
| ABS_CALL |
the call in an absolute analysis that indicates if the transcript was present (P), absent (A), marginal (M), or no call (NC) |
| DETECTION P-VALUE |
'detection p-value', p-value that indicates the significance level of the detection call |
| Data table |
| ID_REF |
VALUE |
ABS_CALL |
DETECTION P-VALUE |
| AFFX-BioB-5_at |
9231.92 |
P |
0.000662269 |
| AFFX-BioB-M_at |
14726.9 |
P |
4.42873e-05 |
| AFFX-BioB-3_at |
10429.1 |
P |
9.4506e-05 |
| AFFX-BioC-5_at |
27940.8 |
P |
7.00668e-05 |
| AFFX-BioC-3_at |
17334.4 |
P |
4.42873e-05 |
| AFFX-BioDn-5_at |
26386.7 |
P |
0.000224668 |
| AFFX-BioDn-3_at |
106242 |
P |
4.42873e-05 |
| AFFX-CreX-5_at |
250690 |
P |
4.42873e-05 |
| AFFX-CreX-3_at |
472216 |
P |
4.42873e-05 |
| AFFX-DapX-5_at |
403911 |
P |
4.42873e-05 |
| AFFX-DapX-M_at |
636114 |
P |
4.42873e-05 |
| AFFX-DapX-3_at |
678868 |
P |
4.42873e-05 |
| AFFX-LysX-5_at |
458915 |
P |
4.42873e-05 |
| AFFX-LysX-M_at |
733267 |
P |
4.42873e-05 |
| AFFX-LysX-3_at |
590801 |
P |
4.42873e-05 |
| AFFX-PheX-5_at |
174381 |
P |
4.42873e-05 |
| AFFX-PheX-M_at |
417072 |
P |
4.42873e-05 |
| AFFX-PheX-3_at |
690943 |
P |
4.42873e-05 |
| AFFX-ThrX-5_at |
19264.5 |
P |
4.42873e-05 |
| AFFX-ThrX-M_at |
153468 |
P |
4.42873e-05 |
Total number of rows: 22575
Table truncated, full table size 666 Kbytes.
| Supplementary file |
Size |
Download |
File type/resource |
| GSM72981.CEL.gz |
2.9 Mb |
(ftp)(http) |
CEL |
| GSM72981.EXP.gz |
498 b |
(ftp)(http) |
EXP |
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