NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Sample GSM730033 Query DataSets for GSM730033
Status Public on Dec 08, 2011
Title P2 fat tissue
Sample type RNA
 
Source name developing adipose tissue
Organism Mus musculus
Characteristics strain: C57BL/6
tissue: developing adipose tissue
developmental stage: P2
Treatment protocol RNA isolated from developing WAT was pooled from four independent embryos at eight different embryonic and early postnatal time points. The RNA was then labeled and hybridized to Illumina Mouse Ref8 BeadChips.
Extracted molecule total RNA
Extraction protocol RNA from each sample was labeled using the Illumina TotalPrep RNA Amplification kit (Ambion) and cRNA was hybridized to Illumina's MouseRef-8 Expression BeadChips (Illumina). The arrays were washed, blocked, and then hybridized. Image processing and data extraction were performed using BeadStudio (Illumina).
Label biotin
Label protocol Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays
 
Hybridization protocol Standard Illumina hybridization protocol
Scan protocol Standard Illumina scanning protocol
Description Developing mouse adipose tissue pooled from at least 3 embryos
Data processing The data were normalised using quantile normalisation with IlluminaGUI in R
 
Submission date May 24, 2011
Last update date Dec 08, 2011
Contact name kivanc birsoy
E-mail(s) kbirsoy@wi.mit.edu
Organization name Rockefeller University
Street address 1230 Rockefeller University
City new york
ZIP/Postal code 10065
Country USA
 
Platform ID GPL6885
Series (1)
GSE29502 Gene expression profile of developing adipose tissue in vivo

Data table header descriptions
ID_REF
VALUE quantile normalized

Data table
ID_REF VALUE
ILMN_1250052 7.247463587
ILMN_3122480 6.799086029
ILMN_2599935 8.054349575
ILMN_2675543 6.897964346
ILMN_2686883 6.919607673
ILMN_2751818 6.814443466
ILMN_2728634 7.07323759
ILMN_3040515 6.868224977
ILMN_2711608 6.851550299
ILMN_1232875 6.880198415
ILMN_1258507 6.888058765
ILMN_2746142 6.826705609
ILMN_1252690 6.921735516
ILMN_2655499 6.773896398
ILMN_1252870 6.986031682
ILMN_1248179 6.802100042
ILMN_2649955 7.327655698
ILMN_2628708 7.307545417
ILMN_3024781 6.845720108
ILMN_2705628 7.244544231

Total number of rows: 25697

Table truncated, full table size 624 Kbytes.




Supplementary data files not provided
Processed data included within Sample table

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap