The adult mouse was injected intraperitoneal (ip) with Escherichia coli lipopolysaccharide (LPS, 0.33mg/kg body weight (BW), ~10ug/mouse; serotype O127:B8, Sigma) and was killed 4 hours after injection by CO2 asphyxiation. Brain was removed, separated in half at the longitudinal fissure, frozen in liquid nitrogen and stored at -80C until use.
Scaling was used: All probe sets, target signal 150. Data parameters: Alpha1 (0.05), Alpha2 (0.065), Tau (0.015), Gamma1L (0.002), Gamma1H (0.002), Gamma2L (0.002667), Gamma2H (0.002667), Perturbation (1.1). Algorithm: Statistical, GCOS 1.1.