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Sample GSM733863 Query DataSets for GSM733863
Status Public on Jul 10, 2011
Title 2008 LAIV subject ID 25 at D7 post-vaccination
Sample type RNA
 
Source name Peripheral blood mononuclear cells of subjects vaccinated with Influenza vaccine at day 0 (before vaccination), and days 3 and 7 post-vaccination.
Organism Homo sapiens
Characteristics cell type: PBMC
subject id: 25
time point: D7
vaccine: LAIV (FluMist, MedImmune)
hai titer (day 0) - a/south dakota/6/2007 (h1n1): 320
hai titer (day 28) - a/south dakota/6/2007 (h1n1): 320
hai titer (day 0) - a/uruguay/716/2007 nymc x-175c (h3n2): 10
hai titer (day 28) - a/uruguay/716/2007 nymc x-175c (h3n2): 20
hai titer (day 0) - b/florida 4/2006: 10
hai titer (day 28) - b/florida 4/2006: 20
sample_import_expsamp_acc: ImmPort:ES497885
Extracted molecule total RNA
Extraction protocol Following PMBC isolation from CPT, 2 x 10^6 cells were lysed in 1 ml of TRIzol and stored at -80C (Cat# 15596-026; Invitrogen Life Technologies). After all time points were collected for a subject, the samples were thawed, and the RNA isolation proceeded according to the manufacturer’s protocol.
Label biotin
Label protocol Total RNA sample quality was evaluated by spectrophotometer to determine quantity, protein contamination and organic solvent contamination, and an Agilent 2100 Bioanalyzer was used to check for RNA degradation. Two-round in vitro transcription amplification and labeling was performed starting with 50 ng intact, total RNA per sample, following the Affymetrix protocol.
 
Hybridization protocol Hybridization was performed on Human U133 Plus 2.0 Arrays (using GeneTitan platform, Affymetrix, or individual cartridges) for 16 h at 45 oC, and 60 r.p.m. in a Hybridization Oven 640 (Affymetrix), slides were washed and stained with a Fluidics Station 450 (Affymetrix).
Scan protocol Scanning was performed on a 7th generation GeneChip Scanner 3000, and the Affymetrix GCOS software was used to perform image analysis and generate raw intensity data.
Description 2008-LAIV-25-D7
Data processing RMA normalization was performed using Expression Console software (Affymetrix Inc, version 1.1).
 
Submission date May 29, 2011
Last update date Feb 25, 2021
Contact name Helder I. Nakaya
E-mail(s) hnakaya@usp.br
Phone +551126481130
Organization name Universidade de São Paulo
Lab csbiology.com
Street address AVENIDA PROFESSOR LINEU PRESTES, 580, Block 17
City SÃO PAULO
State/province SÃO PAULO
ZIP/Postal code 05508000
Country Brazil
 
Platform ID GPL13158
Series (2)
GSE29615 Time Course of Young Adults Vaccinated with Influenza LAIV Vaccine during 2008/09 Flu Season
GSE29619 Systems biology of vaccination for seasonal influenza in humans

Data table header descriptions
ID_REF
VALUE Quantification

Data table
ID_REF VALUE
AFFX-BioB-5_at 4.72408
AFFX-BioB-M_at 6.16233
AFFX-BioB-3_at 5.48108
AFFX-BioC-5_at 7.2481
AFFX-BioC-3_at 7.58869
AFFX-BioDn-5_at 9.03479
AFFX-BioDn-3_at 10.4644
AFFX-CreX-5_at 12.0448
AFFX-CreX-3_at 12.2602
AFFX-DapX-5_at 9.10123
AFFX-DapX-M_at 9.3627
AFFX-DapX-3_at 9.72632
AFFX-LysX-5_at 4.62509
AFFX-LysX-M_at 4.78342
AFFX-LysX-3_at 5.53915
AFFX-PheX-5_at 5.39708
AFFX-PheX-M_at 5.64121
AFFX-PheX-3_at 6.23524
AFFX-ThrX-5_at 6.26392
AFFX-ThrX-M_at 6.88489

Total number of rows: 54715

Table truncated, full table size 1159 Kbytes.




Supplementary file Size Download File type/resource
GSM733863.CEL.gz 2.1 Mb (ftp)(http) CEL
GSM733863.chp.gz 373.3 Kb (ftp)(http) CHP
Processed data included within Sample table
Processed data provided as supplementary file

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