NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Sample GSM733978 Query DataSets for GSM733978
Status Public on Jul 10, 2011
Title 2008 TIV subject ID 46 at D7 post-vaccination
Sample type RNA
 
Source name Peripheral blood mononuclear cells of subjects vaccinated with Influenza vaccine at day 0 (before vaccination), and days 3 and 7 post-vaccination.
Organism Homo sapiens
Characteristics cell type: PBMC
subject id: 46
time point: D7
vaccine: TIV (Fluarix, GlaxoSmithKline Biologicals)
hai titer (day 0) - a/uruguay (h3n2): 320
hai titer (day 28) - a/uruguay (h3n2): 640
hai titer (day 0) - a/brisbane/59/2007 (h1n1): 40
hai titer (day 28) - a/brisbane/59/2007 (h1n1): 80
hai titer (day 0) - b/brisbane/3/2007: 80
hai titer (day 28) - b/brisbane/3/2007: 80
sample_import_expsamp_acc: ImmPort:ES497917
Extracted molecule total RNA
Extraction protocol Following PMBC isolation from CPT, 2 x 10^6 cells were lysed in 1 ml of TRIzol and stored at -80C (Cat# 15596-026; Invitrogen Life Technologies). After all time points were collected for a subject, the samples were thawed, and the RNA isolation proceeded according to the manufacturer’s protocol.
Label biotin
Label protocol Total RNA sample quality was evaluated by spectrophotometer to determine quantity, protein contamination and organic solvent contamination, and an Agilent 2100 Bioanalyzer was used to check for RNA degradation. Two-round in vitro transcription amplification and labeling was performed starting with 50 ng intact, total RNA per sample, following the Affymetrix protocol.
 
Hybridization protocol Hybridization was performed on Human U133 Plus 2.0 Arrays (using GeneTitan platform, Affymetrix, or individual cartridges) for 16 h at 45 oC, and 60 r.p.m. in a Hybridization Oven 640 (Affymetrix), slides were washed and stained with a Fluidics Station 450 (Affymetrix).
Scan protocol Scanning was performed on a 7th generation GeneChip Scanner 3000, and the Affymetrix GCOS software was used to perform image analysis and generate raw intensity data.
Description 2008-TIV-46-D7
Data processing RMA normalization was performed using Expression Console software (Affymetrix Inc, version 1.1).
 
Submission date May 29, 2011
Last update date Feb 25, 2021
Contact name Helder I. Nakaya
E-mail(s) hnakaya@usp.br
Phone +551126481130
Organization name Universidade de São Paulo
Lab csbiology.com
Street address AVENIDA PROFESSOR LINEU PRESTES, 580, Block 17
City SÃO PAULO
State/province SÃO PAULO
ZIP/Postal code 05508000
Country Brazil
 
Platform ID GPL13158
Series (2)
GSE29617 Time Course of Young Adults Vaccinated with Influenza TIV Vaccine during 2008/09 Flu Season
GSE29619 Systems biology of vaccination for seasonal influenza in humans

Data table header descriptions
ID_REF
VALUE Quantification

Data table
ID_REF VALUE
AFFX-BioB-5_at 4.73735
AFFX-BioB-M_at 6.38511
AFFX-BioB-3_at 5.75723
AFFX-BioC-5_at 7.47827
AFFX-BioC-3_at 7.87402
AFFX-BioDn-5_at 9.24623
AFFX-BioDn-3_at 10.8324
AFFX-CreX-5_at 12.1526
AFFX-CreX-3_at 12.3526
AFFX-DapX-5_at 8.91209
AFFX-DapX-M_at 9.2637
AFFX-DapX-3_at 9.79869
AFFX-LysX-5_at 4.5397
AFFX-LysX-M_at 4.42611
AFFX-LysX-3_at 5.5104
AFFX-PheX-5_at 5.12084
AFFX-PheX-M_at 5.5182
AFFX-PheX-3_at 6.51106
AFFX-ThrX-5_at 6.25014
AFFX-ThrX-M_at 6.8269

Total number of rows: 54715

Table truncated, full table size 1159 Kbytes.




Supplementary file Size Download File type/resource
GSM733978.CEL.gz 2.1 Mb (ftp)(http) CEL
GSM733978.chp.gz 373.4 Kb (ftp)(http) CHP
Processed data included within Sample table
Processed data provided as supplementary file

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap