|
| Status |
Public on Jun 07, 2011 |
| Title |
LZS003 |
| Sample type |
RNA |
| |
|
| Source name |
Naïve T cells
|
| Organism |
Mus musculus |
| Characteristics |
cell type: Naïve CD4 T cells
strain: C57BL/6
genotype/variation: wild-type
|
| Treatment protocol |
Two types of cells (wild-type and HIF1a-deficient) were treated under the identifical conditions.
|
| Growth protocol |
Naïve T cells were activated under TH17 conditions for 2.5 days.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted using RNeasy kit (Qiagen).
|
| Label |
Biotin
|
| Label protocol |
Affymetrix standard methods were applied using the high throughput protocal for PM arrays
|
| |
|
| Hybridization protocol |
Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Mouse Genome 430 2.0. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the GeneTitan system
|
| Description |
wild-type TH17 cells
|
| Data processing |
The data were analyzed with Partek Genomics Suite 6.5 using the RMA algorithmn
|
| |
|
| Submission date |
Jun 06, 2011 |
| Last update date |
Jun 07, 2011 |
| Contact name |
David Finkelstein |
| E-mail(s) |
david.finkelstein@stjude.org
|
| Phone |
9014953931
|
| Organization name |
St Jude Children's Research Hospital
|
| Department |
Computational Biology
|
| Street address |
332 N. Lauderdale St.
|
| City |
Memphis |
| State/province |
TN |
| ZIP/Postal code |
38105 |
| Country |
USA |
| |
|
| Platform ID |
GPL11180 |
| Series (1) |
| GSE29765 |
HIF1a-dependent glycolytic pathway orchestrates a metabolic checkpoint for the differentiation of TH17 and Treg cells |
|
