|
Status |
Public on Jun 07, 2011 |
Title |
LZS010 |
Sample type |
RNA |
|
|
Source name |
Naïve T cells
|
Organism |
Mus musculus |
Characteristics |
cell type: Naïve CD4 T cells strain: C57BL/6 genotype/variation: HIF1a-deficient
|
Treatment protocol |
Two types of cells (wild-type and HIF1a-deficient) were treated under the identifical conditions.
|
Growth protocol |
Naïve T cells were activated under TH17 conditions for 2.5 days.
|
Extracted molecule |
total RNA |
Extraction protocol |
RNA was extracted using RNeasy kit (Qiagen).
|
Label |
Biotin
|
Label protocol |
Affymetrix standard methods were applied using the high throughput protocal for PM arrays
|
|
|
Hybridization protocol |
Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Mouse Genome 430 2.0. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
Scan protocol |
GeneChips were scanned using the GeneTitan system
|
Description |
HIF1a-deficient TH17 cells
|
Data processing |
The data were analyzed with Partek Genomics Suite 6.5 using the RMA algorithmn
|
|
|
Submission date |
Jun 06, 2011 |
Last update date |
Jun 07, 2011 |
Contact name |
David Finkelstein |
E-mail(s) |
david.finkelstein@stjude.org
|
Phone |
9014953931
|
Organization name |
St Jude Children's Research Hospital
|
Department |
Computational Biology
|
Street address |
332 N. Lauderdale St.
|
City |
Memphis |
State/province |
TN |
ZIP/Postal code |
38105 |
Country |
USA |
|
|
Platform ID |
GPL11180 |
Series (1) |
GSE29765 |
HIF1a-dependent glycolytic pathway orchestrates a metabolic checkpoint for the differentiation of TH17 and Treg cells |
|