|
| Status |
Public on Jan 17, 2013 |
| Title |
wt E17 hippocampus biological replicate 2 |
| Sample type |
RNA |
| |
|
| Source name |
wt_E17_HPC_biological replicate_2
|
| Organism |
Mus musculus |
| Characteristics |
genotype: wt
age: E17
tissue: hippocampus
genetic background: C57BL/6J
|
| Growth protocol |
Embryoic day 17 and postnatal day 6 littermates were collected
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA were extracted and purified with the miRNeasy Mini-kit (Qiagen).
|
| Label |
biotin
|
| Label protocol |
According to Affymetrix Standard Protocols
|
| |
|
| Hybridization protocol |
According to Affymetrix Standard Protocols
|
| Scan protocol |
According to Affymetrix Standard Protocols
|
| Data processing |
The CEL files were normalized and analyzed using packages in Bioconductor project. The raw intensity data of all PFC samples or HPC samples were first RMA normalized and log2 transformed. differential expression analysis was conducted using limma package.
|
| |
|
| Submission date |
Jun 06, 2011 |
| Last update date |
Jan 17, 2013 |
| Contact name |
Bin XU |
| Organization name |
columbia university
|
| Street address |
1051 Riverside Drive
|
| City |
New York |
| State/province |
NY |
| ZIP/Postal code |
10032 |
| Country |
USA |
| |
|
| Platform ID |
GPL11180 |
| Series (1) |
| GSE29767 |
Gene expression profiles in the hippocampi and prefrontal cortex of Df16(A)+/- mice at embryonic day 17, postnatal day 6, and adult stages |
|
