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Sample GSM738372 Query DataSets for GSM738372
Status Public on Jul 12, 2011
Title wild-type naïve CD4 T cells with CD3-CD28 stimulation for 4h KAI271
Sample type RNA
 
Source name wild-type naïve CD4 T cells with aCD3-CD28 stimulation for 4h
Organism Mus musculus
Characteristics genotype: wild-type
cell type: naive CD4 T cells
treatment: CD3-CD28 stimulation for 4h
genetic background: C57BL\6
Treatment protocol Naïve CD4 and CD8 T cells were sorted from wild-type and HIF1a-deficeint mice. For activation, wild-type and Tsc1-deficient naïve CD4 T cells were stimulated by plate-bound aCD3-CD28 for 4h.
Growth protocol Naïve CD4 and CD8 T cells were cultured with Click medium for aCD3-CD28 stimulation.
Extracted molecule total RNA
Extraction protocol RNA was extracted using RNeasy kit (Qiagen).
Label Biotin
Label protocol Affymetrix standard methods were applied after RNA isolation and amplification
 
Hybridization protocol Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Mouse Genome 430 2.0. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Scan protocol GeneChips were scanned using the GeneChip Scanner 3000 7G.
Description sorted T cells
Data processing The data were analyzed with Partek Genomics Suite 6.5 using the RMA algorithmn
 
Submission date Jun 07, 2011
Last update date Jul 12, 2011
Contact name David Finkelstein
E-mail(s) david.finkelstein@stjude.org
Phone 9014953931
Organization name St Jude Children's Research Hospital
Department Computational Biology
Street address 332 N. Lauderdale St.
City Memphis
State/province TN
ZIP/Postal code 38105
Country USA
 
Platform ID GPL11180
Series (1)
GSE29797 Tuberous sclerosis complex 1 (Tsc1) enforces quiescence of naive T cells to promote immune homeostasis and function

Data table header descriptions
ID_REF
VALUE RMA signal intensity log2 scale

Data table
ID_REF VALUE
1415670_PM_at 8.10321
1415671_PM_at 8.7919
1415672_PM_at 9.51326
1415673_PM_at 6.10863
1415674_PM_a_at 8.59546
1415675_PM_at 7.64981
1415676_PM_a_at 9.93622
1415677_PM_at 4.89042
1415678_PM_at 8.91949
1415679_PM_at 10.2553
1415680_PM_at 8.33002
1415681_PM_at 9.0667
1415682_PM_at 7.07904
1415683_PM_at 9.66448
1415684_PM_at 6.36461
1415685_PM_at 6.8823
1415686_PM_at 7.24451
1415687_PM_a_at 9.03279
1415688_PM_at 9.17207
1415689_PM_s_at 7.44053

Total number of rows: 45141

Table truncated, full table size 983 Kbytes.




Supplementary file Size Download File type/resource
GSM738372_ykr271-htmg430pm.CEL.gz 2.0 Mb (ftp)(http) CEL
Processed data included within Sample table

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