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Sample GSM74052 Query DataSets for GSM74052
Status Public on Sep 13, 2005
Title Ductus Art. 19 days post coitum +O2 chipB
Sample type RNA
 
Source name ductus Arteriosus
Organism Rattus norvegicus
Characteristics Ductus Arteriosus
Extracted molecule total RNA
Label Standard Affymetrix protocol (Affymetrix GeneChip Expression Analysis Technical Manual, rev.3. 2001)
 
Description Isolation of rat DA and Ao. Rats were mated for 24 hours and the end of this period was marked as day zero of pregnancy. Near-term fetuses (gestational age, 19 days) were delivered by Cesarean section from a normoxic or hyperoxic dam, while newborns were used 3 hours after vaginal delivery. Pregnant rats were made hyperoxic to reproduce the neonatal condition in utero. They breathed 100% oxygen inside a box for 3 hours. All animals were killed by cervical dislocation and, to isolate the DA and Ao (arch and thoracic sections), were secured inside a chamber filled with ice-cold Krebs medium through which was bubbled a gas mixture with 2.5 (fetus) or 12.5% (newborn) oxygen. Special care was taken to obtain the blood vessels free of any adhering tissue.
Data processing Original Affymetrix output files
 
Submission date Sep 11, 2005
Last update date Sep 12, 2005
Contact name Flavio Coceani
E-mail(s) coceani@sssup.it
Phone +39 050 883327
Fax +39 050 883215
Organization name Scuola Superiore S. Anna
Department Settore di Scienze Mediche
Street address Piazza Martiri della Libertà, 33
City Pisa
ZIP/Postal code 56127
Country Italy
 
Platform ID GPL86
Series (1)
GSE3290 Expression profiling in perinatal Ductus Arteriosus (DA) and Aorta (Ao).

Data table header descriptions
ID_REF
VALUE Signal
ABS_CALL Detection
DETECTION P-VALUE Detection p-Value

Data table
ID_REF VALUE ABS_CALL DETECTION P-VALUE
AFFX-MurIL2_at 25.7 A 0.860518
AFFX-MurIL10_at 18 A 0.712257
AFFX-MurIL4_at 54.9 A 0.559354
AFFX-MurFAS_at 56.2 A 0.455413
AFFX-BioB-5_at 598.7 P 0.026111
AFFX-BioB-M_at 1026.2 P 0.000127
AFFX-BioB-3_at 565.5 P 0.000662
AFFX-BioC-5_at 2475 P 0.000147
AFFX-BioC-3_at 1732.2 P 0.000095
AFFX-BioDn-5_at 2821.9 P 0.000195
AFFX-BioDn-3_at 9442.1 P 0.000052
AFFX-CreX-5_at 20291.5 P 0.000044
AFFX-CreX-3_at 31631.4 P 0.000044
AFFX-BioB-5_st 84.4 A 0.250777
AFFX-BioB-M_st 79.7 A 0.645547
AFFX-BioB-3_st 32.4 A 0.843268
AFFX-BioC-5_st 42 A 0.897835
AFFX-BioC-3_st 39.3 A 0.58862
AFFX-BioDn-5_st 338.1 A 0.102165
AFFX-BioDn-3_st 109.5 A 0.313723

Total number of rows: 8791

Table truncated, full table size 271 Kbytes.




Supplementary data files not provided

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