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Sample GSM74053 Query DataSets for GSM74053
Status Public on Sep 13, 2005
Title Ductus arteriosus 3hours chip B
Sample type RNA
 
Source name ductus Arteriosus
Organism Rattus norvegicus
Characteristics Ductus Arteriosus
Extracted molecule total RNA
Label Standard Affymetrix protocol (Affymetrix GeneChip Expression Analysis Technical Manual, rev.3. 2001)
 
Description Isolation of rat DA and Ao. Rats were mated for 24 hours and the end of this period was marked as day zero of pregnancy. Near-term fetuses (gestational age, 19 days) were delivered by Cesarean section from a normoxic or hyperoxic dam, while newborns were used 3 hours after vaginal delivery. Pregnant rats were made hyperoxic to reproduce the neonatal condition in utero. They breathed 100% oxygen inside a box for 3 hours. All animals were killed by cervical dislocation and, to isolate the DA and Ao (arch and thoracic sections), were secured inside a chamber filled with ice-cold Krebs medium through which was bubbled a gas mixture with 2.5 (fetus) or 12.5% (newborn) oxygen. Special care was taken to obtain the blood vessels free of any adhering tissue.
Data processing Original Affymetrix output files
 
Submission date Sep 11, 2005
Last update date Sep 12, 2005
Contact name Flavio Coceani
E-mail(s) coceani@sssup.it
Phone +39 050 883327
Fax +39 050 883215
Organization name Scuola Superiore S. Anna
Department Settore di Scienze Mediche
Street address Piazza Martiri della Libertà, 33
City Pisa
ZIP/Postal code 56127
Country Italy
 
Platform ID GPL86
Series (1)
GSE3290 Expression profiling in perinatal Ductus Arteriosus (DA) and Aorta (Ao).

Data table header descriptions
ID_REF
VALUE signal
ABS_CALL Value
DETECTION P-VALUE Detection p-Value

Data table
ID_REF VALUE ABS_CALL DETECTION P-VALUE
AFFX-MurIL2_at 33.3 A 0.804734
AFFX-MurIL10_at 31.2 A 0.645547
AFFX-MurIL4_at 10.7 A 0.868639
AFFX-MurFAS_at 49.7 A 0.699394
AFFX-BioB-5_at 500.1 A 0.083592
AFFX-BioB-M_at 1427.8 P 0.000857
AFFX-BioB-3_at 534.2 P 0.004998
AFFX-BioC-5_at 2183 P 0.000147
AFFX-BioC-3_at 1705 P 0.00034
AFFX-BioDn-5_at 2266.7 P 0.000095
AFFX-BioDn-3_at 15680.2 P 0.00007
AFFX-CreX-5_at 24914.5 P 0.000044
AFFX-CreX-3_at 32252.7 P 0.000044
AFFX-BioB-5_st 155.3 A 0.165861
AFFX-BioB-M_st 153.7 A 0.368438
AFFX-BioB-3_st 123.7 A 0.631562
AFFX-BioC-5_st 36.1 A 0.9273
AFFX-BioC-3_st 12.3 A 0.814869
AFFX-BioDn-5_st 298.1 A 0.131361
AFFX-BioDn-3_st 340.4 A 0.083592

Total number of rows: 8791

Table truncated, full table size 271 Kbytes.




Supplementary data files not provided

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