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Sample GSM74058 Query DataSets for GSM74058
Status Public on Sep 13, 2005
Title Ductus Arteriosus 3hours chip C
Sample type RNA
 
Source name Ductus Arteriosus
Organism Rattus norvegicus
Characteristics ductus arteriosus
Extracted molecule total RNA
Label Standard Affymetrix protocol (Affymetrix GeneChip Expression Analysis Technical Manual, rev.3. 2001)
 
Description Isolation of rat DA and Ao. Rats were mated for 24 hours and the end of this period was marked as day zero of pregnancy. Near-term fetuses (gestational age, 19 days) were delivered by Cesarean section from a normoxic or hyperoxic dam, while newborns were used 3 hours after vaginal delivery. Pregnant rats were made hyperoxic to reproduce the neonatal condition in utero. They breathed 100% oxygen inside a box for 3 hours. All animals were killed by cervical dislocation and, to isolate the DA and Ao (arch and thoracic sections), were secured inside a chamber filled with ice-cold Krebs medium through which was bubbled a gas mixture with 2.5 (fetus) or 12.5% (newborn) oxygen. Special care was taken to obtain the blood vessels free of any adhering tissue.
Data processing Original Affymetrix .dat proprietory output files
 
Submission date Sep 11, 2005
Last update date Sep 12, 2005
Contact name Flavio Coceani
E-mail(s) coceani@sssup.it
Phone +39 050 883327
Fax +39 050 883215
Organization name Scuola Superiore S. Anna
Department Settore di Scienze Mediche
Street address Piazza Martiri della Libertà, 33
City Pisa
ZIP/Postal code 56127
Country Italy
 
Platform ID GPL87
Series (1)
GSE3290 Expression profiling in perinatal Ductus Arteriosus (DA) and Aorta (Ao).

Data table header descriptions
ID_REF
VALUE Signal
ABS_CALL Detection
DETECTION P-VALUE Detection P-value

Data table
ID_REF VALUE ABS_CALL DETECTION P-VALUE
AFFX-MurIL2_at 16.1 A 0.897835
AFFX-MurIL10_at 44 A 0.574038
AFFX-MurIL4_at 9.4 A 0.852061
AFFX-MurFAS_at 19.7 A 0.60308
AFFX-BioB-5_at 548.2 M 0.050229
AFFX-BioB-M_at 1120.3 P 0.000169
AFFX-BioB-3_at 491.2 P 0.000972
AFFX-BioC-5_at 2279.1 P 0.000095
AFFX-BioC-3_at 1700.4 P 0.000446
AFFX-BioDn-5_at 2359.4 P 0.00006
AFFX-BioDn-3_at 15722.9 P 0.00011
AFFX-CreX-5_at 23724.9 P 0.000044
AFFX-CreX-3_at 34690.2 P 0.000044
AFFX-BioB-5_st 261.7 P 0.033677
AFFX-BioB-M_st 141.6 A 0.470241
AFFX-BioB-3_st 61.7 A 0.712257
AFFX-BioC-5_st 21.5 A 0.883887
AFFX-BioC-3_st 9.9 A 0.824672
AFFX-BioDn-5_st 135.1 A 0.287743
AFFX-BioDn-3_st 261.8 A 0.062929

Total number of rows: 8789

Table truncated, full table size 270 Kbytes.




Supplementary data files not provided

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