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Sample GSM74081 Query DataSets for GSM74081
Status Public on Dec 31, 2005
Title Saline treated mouse gallbladder 1
Sample type RNA
Source name gallbladder -saline treated
Organism Mus musculus
Characteristics Strain:B6.V-Lepob
gender: female
age: 12 weeks
tissue: gallbladder
Extracted molecule total RNA
Label biotinylated UTP and CTP
Description Female B6.V-lepob obese mice (n=24) aged 7 weeks were obtained from The Jackson Laboratory (Bar Harbor, ME). Upon arrival, mice were placed on a standard chow diet and were allowed to acclimate fro 1 week before starting the experiment. For leptin treatments, mice received daily intraperitoneal injections of either recombinant mouse leptin (R&D Systems, Minneapolis, MN) (n=12) at a dose of 5 µg/g body weight or with saline as a control (n=12) for 4 weeks. At 12 weeks of age, mice were fasted overnight with free access to water. The following morning the mice underwent cholecystectomy. Three gallbladders were pooled and total RNA was isolated.
Data processing Data were generated using MAS 5.0 and normalized using global scaling.
Submission date Sep 12, 2005
Last update date Oct 28, 2005
Contact name Henry Pitt
Phone 317-2742304
Organization name Indiana University School of Medicine
Street address 535 Barnhill Dr. RT130D
City Indianapolis
State/province IN
ZIP/Postal code 46202-2859
Country USA
Platform ID GPL1261
Series (1)
GSE3293 Leptin Regulated Mouse Gallbladder Genes

Data table header descriptions
VALUE value of the signal
ABS_CALL Detection is absent or present
DETECTION P-VALUE p-value is based on average fluorescent intensity of the array

Data table
AFFX-BioB-5_at 381 P 0.000662
AFFX-BioB-M_at 668.1 P 0.00007
AFFX-BioB-3_at 234.6 P 0.00007
AFFX-BioC-5_at 840.1 P 0.00007
AFFX-BioC-3_at 813.8 P 0.000044
AFFX-BioDn-5_at 975.9 P 0.000044
AFFX-BioDn-3_at 3750.6 P 0.000052
AFFX-CreX-5_at 11427.6 P 0.000052
AFFX-CreX-3_at 12739.7 P 0.000044
AFFX-DapX-5_at 21.7 A 0.313723
AFFX-DapX-M_at 20.1 A 0.440636
AFFX-DapX-3_at 2.5 A 0.981719
AFFX-LysX-5_at 4.2 A 0.574063
AFFX-LysX-M_at 7.5 A 0.921998
AFFX-LysX-3_at 3.7 A 0.39692
AFFX-PheX-5_at 2.8 A 0.957038
AFFX-PheX-M_at 1 A 0.957038
AFFX-PheX-3_at 11 A 0.686277
AFFX-ThrX-5_at 31.1 A 0.262827
AFFX-ThrX-M_at 2.7 A 0.760937

Total number of rows: 45101

Table truncated, full table size 1206 Kbytes.

Supplementary data files not provided

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