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Sample GSM74083 Query DataSets for GSM74083
Status Public on Dec 31, 2005
Title Saline treated mouse gallbladder 2
Sample type RNA
Source name gallbladder saline treated
Organism Mus musculus
Characteristics strain: B6.V-Lep ob
gender: female
age: 12 weeks
tissue: gallbladder
Extracted molecule total RNA
Label biotinylated UTP and CTP
Description Female B6.V-lepob obese mice (n=24) aged 7 weeks were obtained from The Jackson Laboratory (Bar Harbor, ME). Upon arrival, mice were placed on a standard chow diet and were allowed to acclimate fro 1 week before starting the experiment. For leptin treatments, mice received daily intraperitoneal injections of either recombinant mouse leptin (R&D Systems, Minneapolis, MN) (n=12) at a dose of 5 µg/g body weight or with saline as a control (n=12) for 4 weeks. At 12 weeks of age, mice were fasted overnight with free access to water. The following morning the mice underwent cholecystectomy. Three gallbladders were pooled and total RNA was isolated.
Data processing Data were generated using MAS 5.0 and normalized using global scaling.
Submission date Sep 12, 2005
Last update date Oct 28, 2005
Contact name Henry Pitt
Phone 317-2742304
Organization name Indiana University School of Medicine
Street address 535 Barnhill Dr. RT130D
City Indianapolis
State/province IN
ZIP/Postal code 46202-2859
Country USA
Platform ID GPL1261
Series (1)
GSE3293 Leptin Regulated Mouse Gallbladder Genes

Data table header descriptions
VALUE Signal intensity
ABS_CALL Detection is absent or present
DETECTION P-VALUE p-value is based on average fluorescent intensity of the array

Data table
AFFX-BioB-5_at 343.7 P 0.00141
AFFX-BioB-M_at 677 P 0.000258
AFFX-BioB-3_at 320.3 P 0.000258
AFFX-BioC-5_at 850 P 0.00007
AFFX-BioC-3_at 771.2 P 0.000052
AFFX-BioDn-5_at 932.9 P 0.00007
AFFX-BioDn-3_at 3899.1 P 0.000052
AFFX-CreX-5_at 10632.4 P 0.000052
AFFX-CreX-3_at 13314.1 P 0.000044
AFFX-DapX-5_at 10.5 A 0.559354
AFFX-DapX-M_at 47 A 0.195266
AFFX-DapX-3_at 1.9 A 0.994435
AFFX-LysX-5_at 12.1 A 0.340661
AFFX-LysX-M_at 2 A 0.989683
AFFX-LysX-3_at 11.8 A 0.16584
AFFX-PheX-5_at 2.4 A 0.949771
AFFX-PheX-M_at 1.3 A 0.990663
AFFX-PheX-3_at 12 A 0.5
AFFX-ThrX-5_at 6.2 A 0.645547
AFFX-ThrX-M_at 2.9 A 0.897835

Total number of rows: 45101

Table truncated, full table size 1207 Kbytes.

Supplementary data files not provided

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