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Sample GSM74190 Query DataSets for GSM74190
Status Public on Dec 31, 2005
Title Leptin treated mouse gallbladder 5
Sample type RNA
Source name Gallbladder - leptin treated
Organism Mus musculus
Characteristics Strain: B6.V-Lep ob
Gender: Female
Age: 12 weeks
Tissue: Gallbladder
Extracted molecule total RNA
Label biotinylated UTP and CTP
Description Female B6.V-lepob obese mice (n=24) aged 7 weeks were obtained from The Jackson Laboratory (Bar Harbor, ME). Upon arrival, mice were placed on a standard chow diet and were allowed to acclimate fro 1 week before starting the experiment. For leptin treatments, mice received daily intraperitoneal injections of either recombinant mouse leptin (R&D Systems, Minneapolis, MN) (n=12) at a dose of 5 µg/g body weight or with saline as a control (n=12) for 4 weeks. At 12 weeks of age, mice were fasted overnight with free access to water. The following morning the mice underwent cholecystectomy. Three gallbladders were pooled and total RNA was isolated.
Data processing Data were generated using MAS 5.0 and normalized using global scaling.
Submission date Sep 12, 2005
Last update date Oct 28, 2005
Contact name Henry Pitt
Phone 317-2742304
Organization name Indiana University School of Medicine
Street address 535 Barnhill Dr. RT130D
City Indianapolis
State/province IN
ZIP/Postal code 46202-2859
Country USA
Platform ID GPL1261
Series (1)
GSE3293 Leptin Regulated Mouse Gallbladder Genes

Data table header descriptions
VALUE Signal Intensity
ABS_CALL Detection is absent or present
DETECTION P-VALUE p-value is based on average fluorescent intensity of the array

Data table
AFFX-BioB-5_at 319.9 P 0.002023
AFFX-BioB-M_at 399.2 P 0.003212
AFFX-BioB-3_at 179 P 0.003595
AFFX-BioC-5_at 694 P 0.00006
AFFX-BioC-3_at 580.3 P 0.000044
AFFX-BioDn-5_at 653.6 P 0.00007
AFFX-BioDn-3_at 2966.7 P 0.00006
AFFX-CreX-5_at 8790.2 P 0.000044
AFFX-CreX-3_at 10266.5 P 0.000044
AFFX-DapX-5_at 4.5 A 0.39692
AFFX-DapX-M_at 18.5 A 0.440646
AFFX-DapX-3_at 3.2 A 0.997725
AFFX-LysX-5_at 2.7 A 0.910541
AFFX-LysX-M_at 5.4 A 0.883887
AFFX-LysX-3_at 25.3 A 0.300591
AFFX-PheX-5_at 2.5 A 0.960339
AFFX-PheX-M_at 2.6 A 0.86866
AFFX-PheX-3_at 21.4 A 0.686277
AFFX-ThrX-5_at 6.1 A 0.783476
AFFX-ThrX-M_at 22.8 A 0.39692

Total number of rows: 45101

Table truncated, full table size 1209 Kbytes.

Supplementary data files not provided

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