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Sample GSM74192 Query DataSets for GSM74192
Status Public on Dec 31, 2005
Title Leptin treated mouse gallbladder 7
Sample type RNA
 
Source name Gallbladder-leptin treated
Organism Mus musculus
Characteristics Strain: B6.V-Lep ob
Gender: Female
Age: 12 weeks
Tissue: Gallbladder
Extracted molecule total RNA
Label biotinylated UTP and CTP
 
Description Female B6.V-lepob obese mice (n=24) aged 7 weeks were obtained from The Jackson Laboratory (Bar Harbor, ME). Upon arrival, mice were placed on a standard chow diet and were allowed to acclimate fro 1 week before starting the experiment. For leptin treatments, mice received daily intraperitoneal injections of either recombinant mouse leptin (R&D Systems, Minneapolis, MN) (n=12) at a dose of 5 µg/g body weight or with saline as a control (n=12) for 4 weeks. At 12 weeks of age, mice were fasted overnight with free access to water. The following morning the mice underwent cholecystectomy. Three gallbladders were pooled and total RNA was isolated.
Data processing Data were generated using MAS 5.0 and normalized using global scaling.
 
Submission date Sep 12, 2005
Last update date Oct 28, 2005
Contact name Henry Pitt
E-mail(s) hapitt@iupui.edu
Phone 317-2742304
Organization name Indiana University School of Medicine
Street address 535 Barnhill Dr. RT130D
City Indianapolis
State/province IN
ZIP/Postal code 46202-2859
Country USA
 
Platform ID GPL1261
Series (1)
GSE3293 Leptin Regulated Mouse Gallbladder Genes

Data table header descriptions
ID_REF
VALUE Signal intensity
ABS_CALL Detection is absent or present
DETECTION P-VALUE p-value is based on average fluorescent intensity of the array

Data table
ID_REF VALUE ABS_CALL DETECTION P-VALUE
AFFX-BioB-5_at 340.5 P 0.001248
AFFX-BioB-M_at 481.3 P 0.00007
AFFX-BioB-3_at 246.2 P 0.000972
AFFX-BioC-5_at 809.2 P 0.000052
AFFX-BioC-3_at 1027.2 P 0.000044
AFFX-BioDn-5_at 910.8 P 0.000052
AFFX-BioDn-3_at 3417.9 P 0.000044
AFFX-CreX-5_at 10744 P 0.000052
AFFX-CreX-3_at 12835.7 P 0.000044
AFFX-DapX-5_at 65.1 P 0.000857
AFFX-DapX-M_at 40 A 0.327079
AFFX-DapX-3_at 6.3 A 0.891021
AFFX-LysX-5_at 6.3 A 0.559354
AFFX-LysX-M_at 12.4 A 0.737173
AFFX-LysX-3_at 20.8 A 0.205732
AFFX-PheX-5_at 47.3 A 0.51489
AFFX-PheX-M_at 2.1 A 0.976071
AFFX-PheX-3_at 42 A 0.52976
AFFX-ThrX-5_at 18.6 A 0.686277
AFFX-ThrX-M_at 63.7 A 0.108979

Total number of rows: 45101

Table truncated, full table size 1210 Kbytes.




Supplementary data files not provided

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