|
| Status |
Public on May 22, 2012 |
| Title |
Biotin-Only labeled brain samples, 1000ng total RNA, technical rep1 |
| Sample type |
RNA |
| |
|
| Source name |
Brain, 1000ng RNA, biotin-only label
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: brain
rna amount: 1000ng
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was obtained from Ambion FirstChoice Total Brain (Lot number 0906005) and Ambion FirstChoice Total Lung (Lot number 0904002). RNA extraction was performed by Ambion using a proprietary modification of the Ambion AM1910 totally RNA Kit.
|
| Label |
biotin
|
| Label protocol |
The Genisphere FlashTag Biotin Kit was used. The protocol is available for download at the product website (www.genisphere.com) or by contacting the kit manufacturer.
|
| |
|
| Hybridization protocol |
The quantity of labeled extract used varied from 100 to 1000 ng of labeled total RNA (amount of RNA used was an experimental factor). The protocol is available for download from the manufacturer website at www.genisphere.com or www.affymetrix.com.
|
| Scan protocol |
Standard Affymetrix protocol.
|
| Description |
miRNA-1_0 library file used for scanning.
|
| Data processing |
Prior to analysis, raw signal intensity values were Robust Multichip Average (RMA) background corrected, quantile normalized, median polish summarized, and log2 transformed separately for FlashTag biotin-only and FlashTag biotin-HSR chip data.
|
| |
|
| Submission date |
Jun 16, 2011 |
| Last update date |
May 23, 2012 |
| Contact name |
John S. Kaddis |
| E-mail(s) |
jkaddis@coh.org
|
| Organization name |
City of Hope
|
| Department |
Diabetes and Cancer Discovery Science
|
| Street address |
1500 East Duarte Road
|
| City |
Duarte |
| State/province |
CA |
| ZIP/Postal code |
91010-3000 |
| Country |
USA |
| |
|
| Platform ID |
GPL8786 |
| Series (1) |
| GSE30045 |
Influence of RNA Labeling on Expression Profiling of MicroRNAs |
|
