|
| Status |
Public on May 22, 2012 |
| Title |
Biotin-HSR labeled synthetic miRNA spike in, 156.25 attomoles |
| Sample type |
other |
| |
|
| Source name |
Synthetic oligos, 156.25 attomoles
|
| Organism |
synthetic construct |
| Characteristics |
miRNA spike in amount: 156.25 attomoles
|
| Extracted molecule |
other |
| Extraction protocol |
The synthetic miRNAs were obtained from the Ambion mirVana miRNA Reference Panel v9.1 (Lot number 072307).
|
| Label |
biotin-HSR
|
| Label protocol |
The Genisphere FlashTag Biotin-HSR Kit was used. The protocol is available for download at the product website (www.genisphere.com) or by contacting the kit manufacturer.
|
| |
|
| Hybridization protocol |
The quantity of miRNA spike in varied from 5000 to 4.88 attomoles.
|
| Scan protocol |
Standard Affymetrix protocol.
|
| Description |
Synthetic human, rat, and mouse miRNAs.
miRNA-1_0_2Xgain library file used for scanning.
|
| Data processing |
Prior to analysis, raw signal intensity values were Robust Multichip Average (RMA) background corrected, quantile normalized, median polish summarized, and log2 transformed separately for FlashTag biotin-only and FlashTag biotin-HSR chip data.
|
| |
|
| Submission date |
Jun 16, 2011 |
| Last update date |
May 23, 2012 |
| Contact name |
John S. Kaddis |
| E-mail(s) |
jkaddis@coh.org
|
| Organization name |
City of Hope
|
| Department |
Diabetes and Cancer Discovery Science
|
| Street address |
1500 East Duarte Road
|
| City |
Duarte |
| State/province |
CA |
| ZIP/Postal code |
91010-3000 |
| Country |
USA |
| |
|
| Platform ID |
GPL8786 |
| Series (1) |
| GSE30045 |
Influence of RNA Labeling on Expression Profiling of MicroRNAs |
|
