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Status |
Public on May 22, 2012 |
Title |
Biotin-HSR labeled synthetic miRNA spike in, 625 attomoles |
Sample type |
other |
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Source name |
Synthetic oligos, 625 attomoles
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Organism |
synthetic construct |
Characteristics |
miRNA spike in amount: 625 attomoles
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Extracted molecule |
other |
Extraction protocol |
The synthetic miRNAs were obtained from the Ambion mirVana miRNA Reference Panel v9.1 (Lot number 072307).
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Label |
biotin-HSR
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Label protocol |
The Genisphere FlashTag Biotin-HSR Kit was used. The protocol is available for download at the product website (www.genisphere.com) or by contacting the kit manufacturer.
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Hybridization protocol |
The quantity of miRNA spike in varied from 5000 to 4.88 attomoles.
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Scan protocol |
Standard Affymetrix protocol.
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Description |
Synthetic human, rat, and mouse miRNAs. miRNA-1_0_2Xgain library file used for scanning.
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Data processing |
Prior to analysis, raw signal intensity values were Robust Multichip Average (RMA) background corrected, quantile normalized, median polish summarized, and log2 transformed separately for FlashTag biotin-only and FlashTag biotin-HSR chip data.
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Submission date |
Jun 16, 2011 |
Last update date |
May 23, 2012 |
Contact name |
John S. Kaddis |
E-mail(s) |
jkaddis@coh.org
|
Organization name |
City of Hope
|
Department |
Diabetes and Cancer Discovery Science
|
Street address |
1500 East Duarte Road
|
City |
Duarte |
State/province |
CA |
ZIP/Postal code |
91010-3000 |
Country |
USA |
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|
Platform ID |
GPL8786 |
Series (1) |
GSE30045 |
Influence of RNA Labeling on Expression Profiling of MicroRNAs |
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