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Sample GSM745456 Query DataSets for GSM745456
Status Public on Aug 31, 2011
Title 9171_MEF
Sample type RNA
 
Source name MEFs
Organism Mus musculus
Characteristics background strain: a mixed background C57BL/6;129S
cell type: uninfected MEF
Treatment protocol TetO-FUW-based lentiviruses were prepared as previously described with some modification for hepatocytes.
Growth protocol cells grow in standard hepatocyte culturing media or neuronal medium.
Extracted molecule total RNA
Extraction protocol RNA was extracted with Trizol reagent, followed by clean-up and DNase I treatment with QIAGEN RNeasy mini kit in accordance with the prescribed protocol provided with the kit. Quality control was performed with Agilent Bioanalyser.
Label biotin
Label protocol Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays.
 
Hybridization protocol Standard Illumina hybridization protocol
Scan protocol Standard Illumina scanning protocol
Description uninfected MEF passage 3
Data processing The data were normalised using quantile normalisation with IlluminaGUI in R
 
Submission date Jun 20, 2011
Last update date Aug 31, 2011
Contact name Miao-Chih Tsai
E-mail(s) miaochih@gmail.com
Organization name Stanford University
Street address 269 Campus Drive, CCSR 2140
City Stanford
State/province CA
ZIP/Postal code 94305
Country USA
 
Platform ID GPL6885
Series (1)
GSE30102 Direct lineage conversion of terminally differentiated hepatocytes to functional neurons

Data table header descriptions
ID_REF
VALUE quantile normalized
Detection Pval

Data table
ID_REF VALUE Detection Pval
ILMN_1248788 43.66434 0.3383459
ILMN_2707227 66.20885 0.01002506
ILMN_2896528 1298.724 0
ILMN_2721178 297.9998 0
ILMN_1227723 212.4818 0
ILMN_3033922 672.0844 0
ILMN_3092673 2437.336 0
ILMN_2730714 2470.51 0
ILMN_3162224 1115.718 0
ILMN_2816356 83.18051 0.003759399
ILMN_2808939 920.9901 0
ILMN_2634564 376.4534 0
ILMN_1216623 520.5145 0
ILMN_1215157 606.2332 0
ILMN_1228777 28.10185 0.9586466
ILMN_2737647 36.20893 0.6804512
ILMN_1216425 37.85213 0.5927318
ILMN_2734484 360.1031 0
ILMN_2652961 32.0089 0.8709273
ILMN_2952292 115.442 0

Total number of rows: 25697

Table truncated, full table size 733 Kbytes.




Supplementary data files not provided
Raw data are available on Series record
Processed data included within Sample table

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