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Sample GSM745458 Query DataSets for GSM745458
Status Public on Aug 31, 2011
Title 9176_neuron
Sample type RNA
 
Source name cortical neurons
Organism Mus musculus
Characteristics background strain: a mixed background C57BL/6;129S
cell type: cortical neuron
Treatment protocol TetO-FUW-based lentiviruses were prepared as previously described with some modification for hepatocytes.
Growth protocol cells grow in standard hepatocyte culturing media or neuronal medium.
Extracted molecule total RNA
Extraction protocol RNA was extracted with Trizol reagent, followed by clean-up and DNase I treatment with QIAGEN RNeasy mini kit in accordance with the prescribed protocol provided with the kit. Quality control was performed with Agilent Bioanalyser.
Label biotin
Label protocol Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays.
 
Hybridization protocol Standard Illumina hybridization protocol
Scan protocol Standard Illumina scanning protocol
Description Postnatal cortical neurons derived from TauGFP mice, cultured for 13 days and sorted for GFP+ cells before RNA extraction
Data processing The data were normalised using quantile normalisation with IlluminaGUI in R
 
Submission date Jun 20, 2011
Last update date Aug 31, 2011
Contact name Miao-Chih Tsai
E-mail(s) miaochih@gmail.com
Organization name Stanford University
Street address 269 Campus Drive, CCSR 2140
City Stanford
State/province CA
ZIP/Postal code 94305
Country USA
 
Platform ID GPL6885
Series (1)
GSE30102 Direct lineage conversion of terminally differentiated hepatocytes to functional neurons

Data table header descriptions
ID_REF
VALUE quantile normalized
Detection Pval

Data table
ID_REF VALUE Detection Pval
ILMN_1248788 24.52452 0.7067669
ILMN_2707227 48.9982 0.03383459
ILMN_2896528 2748.235 0
ILMN_2721178 736.3309 0
ILMN_1227723 359.6666 0
ILMN_3033922 1707.858 0
ILMN_3092673 5014.373 0
ILMN_2730714 1902.935 0
ILMN_3162224 1416.203 0
ILMN_2816356 56.88736 0.01002506
ILMN_2808939 524.452 0
ILMN_2634564 402.2192 0
ILMN_1216623 566.6284 0
ILMN_1215157 721.4594 0
ILMN_1228777 30.11302 0.3847118
ILMN_2737647 31.30708 0.3383459
ILMN_1216425 21.79096 0.8646616
ILMN_2734484 862.3097 0
ILMN_2652961 23.14737 0.8007519
ILMN_2952292 83.98068 0

Total number of rows: 25697

Table truncated, full table size 725 Kbytes.




Supplementary data files not provided
Raw data are available on Series record
Processed data included within Sample table

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