|
| Status |
Public on Aug 31, 2011 |
| Title |
9178_BAM |
| Sample type |
RNA |
| |
|
| Source name |
MEFs with BAM
|
| Organism |
Mus musculus |
| Characteristics |
background strain: a mixed background C57BL/6;129S
cell type: MEF-iN cells (MEF derived induced neurons)
|
| Treatment protocol |
TetO-FUW-based lentiviruses were prepared as previously described with some modification for hepatocytes.
|
| Growth protocol |
cells grow in standard hepatocyte culturing media or neuronal medium.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted with Trizol reagent, followed by clean-up and DNase I treatment with QIAGEN RNeasy mini kit in accordance with the prescribed protocol provided with the kit. Quality control was performed with Agilent Bioanalyser.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays.
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
MEF-iN cells (MEF derived induced neurons) derived from TauGFP embryos 13 days after infection with the 3 factors (Ascl1 -Brn2 - Myt1l) and sorted for GFP+ cells before RNA extraction.
|
| Data processing |
The data were normalised using quantile normalisation with IlluminaGUI in R
|
| |
|
| Submission date |
Jun 20, 2011 |
| Last update date |
Aug 31, 2011 |
| Contact name |
Miao-Chih Tsai |
| E-mail(s) |
miaochih@gmail.com
|
| Organization name |
Stanford University
|
| Street address |
269 Campus Drive, CCSR 2140
|
| City |
Stanford |
| State/province |
CA |
| ZIP/Postal code |
94305 |
| Country |
USA |
| |
|
| Platform ID |
GPL6885 |
| Series (1) |
| GSE30102 |
Direct lineage conversion of terminally differentiated hepatocytes to functional neurons |
|
