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Sample GSM745460 Query DataSets for GSM745460
Status Public on Aug 31, 2011
Title 9178_BAM
Sample type RNA
 
Source name MEFs with BAM
Organism Mus musculus
Characteristics background strain: a mixed background C57BL/6;129S
cell type: MEF-iN cells (MEF derived induced neurons)
Treatment protocol TetO-FUW-based lentiviruses were prepared as previously described with some modification for hepatocytes.
Growth protocol cells grow in standard hepatocyte culturing media or neuronal medium.
Extracted molecule total RNA
Extraction protocol RNA was extracted with Trizol reagent, followed by clean-up and DNase I treatment with QIAGEN RNeasy mini kit in accordance with the prescribed protocol provided with the kit. Quality control was performed with Agilent Bioanalyser.
Label biotin
Label protocol Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays.
 
Hybridization protocol Standard Illumina hybridization protocol
Scan protocol Standard Illumina scanning protocol
Description MEF-iN cells (MEF derived induced neurons) derived from TauGFP embryos 13 days after infection with the 3 factors (Ascl1 -Brn2 - Myt1l) and sorted for GFP+ cells before RNA extraction.
Data processing The data were normalised using quantile normalisation with IlluminaGUI in R
 
Submission date Jun 20, 2011
Last update date Aug 31, 2011
Contact name Miao-Chih Tsai
E-mail(s) miaochih@gmail.com
Organization name Stanford University
Street address 269 Campus Drive, CCSR 2140
City Stanford
State/province CA
ZIP/Postal code 94305
Country USA
 
Platform ID GPL6885
Series (1)
GSE30102 Direct lineage conversion of terminally differentiated hepatocytes to functional neurons

Data table header descriptions
ID_REF
VALUE quantile normalized
Detection Pval

Data table
ID_REF VALUE Detection Pval
ILMN_1248788 26.57074 0.9197995
ILMN_2707227 49.52137 0.04511278
ILMN_2896528 2132.154 0
ILMN_2721178 494.3358 0
ILMN_1227723 182.8075 0
ILMN_3033922 1621.938 0
ILMN_3092673 6054.066 0
ILMN_2730714 3196.971 0
ILMN_3162224 1185.857 0
ILMN_2816356 84.80584 0
ILMN_2808939 498.689 0
ILMN_2634564 422.8564 0
ILMN_1216623 576.0591 0
ILMN_1215157 626.7879 0
ILMN_1228777 30.34272 0.7606516
ILMN_2737647 54.91006 0.01754386
ILMN_1216425 31.90588 0.6679198
ILMN_2734484 843.6553 0
ILMN_2652961 30.51402 0.7543859
ILMN_2952292 212.2722 0

Total number of rows: 25697

Table truncated, full table size 720 Kbytes.




Supplementary data files not provided
Raw data are available on Series record
Processed data included within Sample table

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