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Sample GSM745461 Query DataSets for GSM745461
Status Public on Aug 31, 2011
Title 9179_BAM_ATT
Sample type RNA
 
Source name Hepatocyte with BAM
Organism Mus musculus
Characteristics background strain: a mixed background C57BL/6;129S
cell type: Hep-iN cells (Hepatocytes derived induced neurons) 
Treatment protocol TetO-FUW-based lentiviruses were prepared as previously described with some modification for hepatocytes.
Growth protocol cells grow in standard hepatocyte culturing media or neuronal medium.
Extracted molecule total RNA
Extraction protocol RNA was extracted with Trizol reagent, followed by clean-up and DNase I treatment with QIAGEN RNeasy mini kit in accordance with the prescribed protocol provided with the kit. Quality control was performed with Agilent Bioanalyser.
Label biotin
Label protocol Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays.
 
Hybridization protocol Standard Illumina hybridization protocol
Scan protocol Standard Illumina scanning protocol
Description Hep-iN cells (Hepatocytes derived induced neurons) derived from TauGFP-AlbCre-R26-lsl-tomato (13 days after infection with the 3 factors (Ascl1 - Brn2 - Myt1l) double sorted for tdTomato+GFP+ cells before RNA extraction.
Data processing The data were normalised using quantile normalisation with IlluminaGUI in R
 
Submission date Jun 20, 2011
Last update date Aug 31, 2011
Contact name Miao-Chih Tsai
E-mail(s) miaochih@gmail.com
Organization name Stanford University
Street address 269 Campus Drive, CCSR 2140
City Stanford
State/province CA
ZIP/Postal code 94305
Country USA
 
Platform ID GPL6885
Series (1)
GSE30102 Direct lineage conversion of terminally differentiated hepatocytes to functional neurons

Data table header descriptions
ID_REF
VALUE quantile normalized
Detection Pval

Data table
ID_REF VALUE Detection Pval
ILMN_1248788 11.54346 0.4674186
ILMN_2707227 296.4684 0
ILMN_2896528 2127.843 0
ILMN_2721178 377.5081 0
ILMN_1227723 231.0284 0
ILMN_3033922 961.207 0
ILMN_3092673 4312.514 0
ILMN_2730714 3880.893 0
ILMN_3162224 765.687 0
ILMN_2816356 39.03715 0.01002506
ILMN_2808939 460.7608 0
ILMN_2634564 387.9026 0
ILMN_1216623 530.8448 0
ILMN_1215157 582.6911 0
ILMN_1228777 5.031166 0.7656642
ILMN_2737647 24.53962 0.08145364
ILMN_1216425 11.81825 0.4473684
ILMN_2734484 521.4778 0
ILMN_2652961 5.517117 0.7531328
ILMN_2952292 145.6336 0

Total number of rows: 25697

Table truncated, full table size 721 Kbytes.




Supplementary data files not provided
Raw data are available on Series record
Processed data included within Sample table

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