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Sample GSM74983 Query DataSets for GSM74983
Status Public on Sep 21, 2005
Title T24 caspase activation time
Sample type RNA
 
Channel 1
Source name Treated T24 cell line
Organism Homo sapiens
Characteristics T24 cell line treated with 2,4-dibencilaminoquinazoline for 20 hours
Biomaterial provider ATCC
Extracted molecule total RNA
Label Cy5
 
Channel 2
Source name Control T24 cell line
Organism Homo sapiens
Characteristics T24 cell line not treated with 2,4-dibencilaminoquinazoline (negative control).
Extracted molecule total RNA
Label Cy3
 
 
Description Treatment of T24 cells with 2,4-dibencilaminoquinazoline during the time necessary to induce caspase activation(20 hours).
Data processing The hybridized slides were scanned with the GMS 418 scanner (Genetic Microsystems, Woburn, MA),. After image acquisition, the scanned images were imported into “ImaGene 4.1” software (BioDiscovery) to quantify the signal intensities. Data from spots not recognized by the Imagene analysis software were excluded from further considerations (empty, poor and negative spots). We also removed data from spots identified as visually flawed (<15% in all arrays). The fluorescent median signal intensity for each spot was calculated using local median background subtraction. Data were normalized using Global mean, Dye-swap Pairs normalization and Dye Swap Fix filter 1.5 with the program ArrayNorm 1.7 (Graz, Austria)42. Log ratios above 1,7 or below -1,7 were considered as differential expression.
 
Submission date Sep 19, 2005
Last update date Oct 28, 2005
Contact name Raquel Malumbres
E-mail(s) rmalumbres@yahoo.com
Phone +34 948194700
Organization name University of Navarra/ Clínica Universidad de Navarra
Department Hemato-Oncology
Lab Multiple Myeloma
Street address Avenida Pio XII 55
City Pamplona
State/province Navarra
ZIP/Postal code 31008
Country Spain
 
Platform ID GPL2006
Series (1)
GSE3329 2,4-dibencilaminoquinazoline induced apoptosis in three human cancer cell lines

Data table header descriptions
ID_REF
VALUE Normalized log ratio (Dye swap pairs normalization)
CH1_SIG_MEAN Signal intensity value of the treated sample
CH1_BKD_MEAN Background intensity value of the treated sample
CH2_SIG_MEAN Signal intensity value of the control sample
CH2_BKD_MEAN Background intensity value of the control sample

Data table
ID_REF VALUE CH1_SIG_MEAN CH1_BKD_MEAN CH2_SIG_MEAN CH2_BKD_MEAN
1 -0.052692134 2055.014493 427.678392 1462.84058 525.0050251
2 -0.4549502 1261.478261 474.8699552 1412.405797 513.3991031
3 -0.15511669 19889.30435 505.9461883 10397.7971 572.2511211
4 -0.1781046 2155.217391 480.3766816 1627.536232 539.6950673
5 -0.11697624 9950.724638 495.6847291 5476.26087 473.2315271
6 -0.37719163 6158.231884 518.7881773 3625.710145 528.7881773
7 -0.12955418 44653.43478 462.9147982 9917.362319 444.2869955
8 2927.710145 434.0807175 2077.217391 478.2869955
9 -0.14712155 3032.608696 457.3004926 1955.681159 470.137931
10 -0.1647494 1865.275362 451.9704433 1396.637681 455.6256158
11 -0.20167515 2353.188406 537.7241379 1652.898551 502.1083744
12 -0.32906547 6045.449275 527.6847291 3698.666667 508.08867
13 -0.25043926 2479.971014 542.5219941 1802.231884 519.4545455
14 0.14769498 1467.536232 484.0269058 1167.275362 604.6188341
15 0.29546094 1365.971014 470.4315353 1174.753623 529.8921162
16 7252.463768 439.9704433 4261.623188 473.6157635
17 0.004706702 1199.014493 401.1800766 774.5797101 429.6168582
18 862.057971 429.7488789 1014.463768 524.4753363
19 -0.38335446 4095.855072 523.7873754 3399.942029 656.6179402
20 0.5911025 859.884058 470.7551867 987.5942029 552.9875519

Total number of rows: 19200

Table truncated, full table size 1161 Kbytes.




Supplementary file Size Download File type/resource
GSM74983-cy3-IMAGENE.txt.gz 1.0 Mb (ftp)(http) TXT
GSM74983-cy5-IMAGENE.txt.gz 1.0 Mb (ftp)(http) TXT

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