|
Status |
Public on Jun 14, 2013 |
Title |
antibody mediated rejection 94 |
Sample type |
RNA |
|
|
Source name |
kidney allograft biopsy
|
Organism |
Homo sapiens |
Characteristics |
tissue: kidney sample type: acute humoral rejection
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA from ten slides (5 µm) was isolated and purified using miRNeasy FFPE Kit (Qiagen, Cat.No. 217404)
|
Label |
biotin
|
Label protocol |
Total RNA (1µg per sample) was labeled using the FlashTag RNA Labeling Kit (Genisphere, Cat.# FT30AFYB) and hybridized to the arrays as described by the manufacturer (Affymetrix, Santa Clara, CA).
|
|
|
Hybridization protocol |
The complementary RNA hybridization cocktail was incubated overnight at 48°C while rotating in a hybridization oven. After 16 hours of hybridization, the cocktail was removed and the arrays were washed and stained in an Affymetrix GeneChip fluidics station 450.
|
Scan protocol |
Arrays were scanned on an Affymetrix GeneChip 3000G scanner.
|
Description |
miRNA profile of acute humoral rejection
|
Data processing |
All ouput files (.cel) were analyzed by the miRNA QC Tool (Affymetrix) to assess data quality. Affymetrix data were preprocessed, normalized, summarized and annotated with the robust multi-average (RMA) method, quantile normalization and corresponding annotation file (.cdf). Only miRNAs with at least 80% of valid entries were considered for successive analysis steps
|
|
|
Submission date |
Jun 28, 2011 |
Last update date |
Jun 15, 2013 |
Contact name |
Julia Wilflingseder |
E-mail(s) |
julia.wilflingseder@meduniwien.ac.at
|
Organization name |
Medical University of Vienna
|
Department |
Nephrology and Dialysis
|
Lab |
Laboratory Rainer Oberbauer
|
Street address |
Währinger Gürtel 18-20
|
City |
Vienna |
ZIP/Postal code |
1090 |
Country |
Austria |
|
|
Platform ID |
GPL8786 |
Series (1) |
GSE30282 |
miRNAs in renal transplantation |
|