|
| Status |
Public on Jun 14, 2013 |
| Title |
acute cellular rejection, BANFF1 25 |
| Sample type |
RNA |
| |
|
| Source name |
kidney allograft biopsy
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: kidney
sample type: acute cellular rejection
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA from ten slides (5 µm) was isolated and purified using miRNeasy FFPE Kit (Qiagen, Cat.No. 217404)
|
| Label |
biotin
|
| Label protocol |
Total RNA (1µg per sample) was labeled using the FlashTag RNA Labeling Kit (Genisphere, Cat.# FT30AFYB) and hybridized to the arrays as described by the manufacturer (Affymetrix, Santa Clara, CA).
|
| |
|
| Hybridization protocol |
The complementary RNA hybridization cocktail was incubated overnight at 48°C while rotating in a hybridization oven. After 16 hours of hybridization, the cocktail was removed and the arrays were washed and stained in an Affymetrix GeneChip fluidics station 450.
|
| Scan protocol |
Arrays were scanned on an Affymetrix GeneChip 3000G scanner.
|
| Description |
miRNA profile of acute cellular rejection
|
| Data processing |
All ouput files (.cel) were analyzed by the miRNA QC Tool (Affymetrix) to assess data quality. Affymetrix data were preprocessed, normalized, summarized and annotated with the robust multi-average (RMA) method, quantile normalization and corresponding annotation file (.cdf). Only miRNAs with at least 80% of valid entries were considered for successive analysis steps
|
| |
|
| Submission date |
Jun 28, 2011 |
| Last update date |
Jun 15, 2013 |
| Contact name |
Julia Wilflingseder |
| E-mail(s) |
julia.wilflingseder@meduniwien.ac.at
|
| Organization name |
Medical University of Vienna
|
| Department |
Nephrology and Dialysis
|
| Lab |
Laboratory Rainer Oberbauer
|
| Street address |
Währinger Gürtel 18-20
|
| City |
Vienna |
| ZIP/Postal code |
1090 |
| Country |
Austria |
| |
|
| Platform ID |
GPL8786 |
| Series (1) |
| GSE30282 |
miRNAs in renal transplantation |
|
