|
Status |
Public on Oct 15, 2011 |
Title |
717-A4 |
Sample type |
other |
|
|
Source name |
HCC from explanted liver at time of transplant
|
Organism |
Homo sapiens |
Characteristics |
tissue: hepatocellular carcinoma recurrence status: Non-Recurrence milan criteria: Outside
|
Treatment protocol |
Patients received liver transplants for HCC. Recurrent HCC within 3 years of transplant and Milan criteria status at time of transplant were noted for each patient.
|
Extracted molecule |
other |
Extraction protocol |
Micro RNA extraction from FFPE tissue blocks using the Roche High Pure miRNA Isolation kit per the manufacturer's instructions.
|
Label |
biotin
|
Label protocol |
MiRNA samples were labeled with the Genisphere FlashTag Biotin labeling kit per the manufacturer's instructions.
|
|
|
Hybridization protocol |
Labeled samples were hybridized to Affymetrix micro RNA 1.0 microarrays per the manufacturer's instructions.
|
Scan protocol |
Hybridized arrays were scanned with an Affymetrix GenChip Scanner 7G Plus.
|
Description |
Micro RNA expression from liver transplant patients with HCC
|
Data processing |
CEL files were generated using the miRNA-1_0_2X gain library file. Hybridization quality metrics were assessed using the AffyMir miRNA QCTool program using RMA normalization.
|
|
|
Submission date |
Jun 29, 2011 |
Last update date |
Oct 15, 2011 |
Contact name |
Christopher Taylor Barry |
E-mail(s) |
chris_barry@urmc.rochester.edu
|
Phone |
585-705-6703
|
Fax |
585-271-7929
|
URL |
http://www.livercancergenomics.com
|
Organization name |
University of Rochester
|
Department |
Transplant Surgery
|
Lab |
Barry
|
Street address |
601 Elmwood Avenue
|
City |
Rochester |
State/province |
NY |
ZIP/Postal code |
14642 |
Country |
USA |
|
|
Platform ID |
GPL8786 |
Series (1) |
GSE30297 |
Micro RNA expression from human hepatocellular carcinoma (HCC) specimens from patients undergoing liver transplantation. |
|