|
| Status |
Public on Oct 15, 2011 |
| Title |
735 |
| Sample type |
other |
| |
|
| Source name |
HCC from explanted liver at time of transplant
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: hepatocellular carcinoma
recurrence status: Recurrence
milan criteria: Within
|
| Treatment protocol |
Patients received liver transplants for HCC. Recurrent HCC within 3 years of transplant and Milan criteria status at time of transplant were noted for each patient.
|
| Extracted molecule |
other |
| Extraction protocol |
Micro RNA extraction from FFPE tissue blocks using the Roche High Pure miRNA Isolation kit per the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
MiRNA samples were labeled with the Genisphere FlashTag Biotin labeling kit per the manufacturer's instructions.
|
| |
|
| Hybridization protocol |
Labeled samples were hybridized to Affymetrix micro RNA 1.0 microarrays per the manufacturer's instructions.
|
| Scan protocol |
Hybridized arrays were scanned with an Affymetrix GenChip Scanner 7G Plus.
|
| Description |
Micro RNA expression from liver transplant patients with HCC
|
| Data processing |
CEL files were generated using the miRNA-1_0_2X gain library file. Hybridization quality metrics were assessed using the AffyMir miRNA QCTool program using RMA normalization.
|
| |
|
| Submission date |
Jun 29, 2011 |
| Last update date |
Oct 15, 2011 |
| Contact name |
Christopher Taylor Barry |
| E-mail(s) |
chris_barry@urmc.rochester.edu
|
| Phone |
585-705-6703
|
| Fax |
585-271-7929
|
| URL |
http://www.livercancergenomics.com
|
| Organization name |
University of Rochester
|
| Department |
Transplant Surgery
|
| Lab |
Barry
|
| Street address |
601 Elmwood Avenue
|
| City |
Rochester |
| State/province |
NY |
| ZIP/Postal code |
14642 |
| Country |
USA |
| |
|
| Platform ID |
GPL8786 |
| Series (1) |
| GSE30297 |
Micro RNA expression from human hepatocellular carcinoma (HCC) specimens from patients undergoing liver transplantation. |
|
