|
| Status |
Public on Jul 07, 2011 |
| Title |
blood 0.5g T25 ppB |
| Sample type |
RNA |
| |
|
| Source name |
blood 0.5g T25 ppB
|
| Organism |
Homo sapiens |
| Characteristics |
donor: B
Sex: M
tissue: blood
treatment: 0.5g APAP exposure
time: 25h
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated using the PAXgene Blood RNA kit (PreAnalytiX/Qiagen) according to the manufacturers protocol (including Dnase) and was globin reduced using the GLOBINclear kit (Ambion) according to the manufacturers protocol
|
| Label |
biotin
|
| Label protocol |
We used standard Affymetrix labeling protocols
|
| |
|
| Hybridization protocol |
We used standard Affymetrix hybridisation rotocols
|
| Scan protocol |
We used standard Affymetrix scanning parameters
|
| Description |
globin reduced total RNA
sample 0.5g APAP exposure
|
| Data processing |
All samples were normalized by RMA algorithm and analyzed by SAM (FC>1.5 and FDR<10%).
|
| |
|
| Submission date |
Jul 05, 2011 |
| Last update date |
Jul 07, 2011 |
| Contact name |
marlon jetten |
| E-mail(s) |
marlon.jetten@maastrichtuniversity.nl
|
| Organization name |
maastricht university
|
| Street address |
universiteitssingel 50
|
| City |
Maastricht |
| ZIP/Postal code |
6200MD |
| Country |
Netherlands |
| |
|
| Platform ID |
GPL10526 |
| Series (1) |
|
