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Sample GSM754746 Query DataSets for GSM754746
Status Public on Nov 28, 2011
Title cardiac bodies, rep 2 [MoGene[exon_level]]
Sample type RNA
 
Source name cardiac bodies
Organism Mus musculus
Characteristics age: 14 day
tissue/cell type: cardiac bodies (cell culture)
Growth protocol Mouse embryonic stem cells are differentiated into cardiomyocytes with DMEM based culture media supplied with 10% serum. Mouse hearts dissected from E17.5 and P1 mice.
Extracted molecule total RNA
Extraction protocol RNA was extracted from each samples (culture cells and tissues) with TRIzol reagent (Invitrogen). Isolated RNAs were then treated with the RiobMinus rRNA reduction kit (Invitrogen), cDNA synthesis and amplification was performed using the WT cDNA synthesis and amplification kit (Affymetrix).
Label biotin
Label protocol Samples were enzymatically fragmented and biotinylated using the WT Terminal Labeling Kit (Affymetrix)
 
Hybridization protocol Samples were hybridized using Affymetrix hybridization kit materials. Heat cocktails at 99C for 5 minutes, then 42C for 5 minutes centrifuge at max speed for 1 minute, Transfer 200ul of hyb solution to each array, then tape holes, Hybridize 17 hours at 45C at 60rpm. Fluidics washing is FS450_0001.
Scan protocol Affymetrix Gene ChIP Scanner 3000 7G
Description cardiac bodies
Data processing RMA normalized, gene and exon level expression values from Affymetrix Power Tools
MoGene-1_0-st-v1.r4.pgf
MoGene-1_0-st-v1.r4.mps and MoGene-1_0-st-v1.r4.all.ps
 
Submission date Jul 06, 2011
Last update date Nov 29, 2011
Contact name Pascal Gellert
E-mail(s) pascal.gellert@mpi-bn.mpg.de
Phone +49 6032 705 1765
URL http://www.mpi-hlr.de
Organization name Max Planck Institut for Heart and Lung Research
Department Cardiac Development and Remodelling
Lab Braun
Street address Ludwigstr. 43
City Bad Nauheim
ZIP/Postal code 61231
Country Germany
 
Platform ID GPL10740
Series (2)
GSE30429 Gene Array Analyzer (GAA): Alternative usage of gene arrays to study alternative splicing events (MoGene array)
GSE33183 Gene Array Analyzer (GAA): Alternative usage of gene arrays to study alternative splicing events
Relations
Alternative to GSM754756 (gene-level analysis)

Data table header descriptions
ID_REF
VALUE RMA normalized

Data table
ID_REF VALUE
10338001 11.90561
10338002 6.78616
10338003 10.04533
10338004 9.26068
10338005 1.94009
10338006 2.23473
10338007 2.69926
10338008 3.81265
10338009 8.94649
10338010 1.97117
10338011 6.17734
10338012 2.10494
10338013 1.75446
10338014 1.85854
10338015 1.80638
10338016 8.26394
10338017 13.17307
10338018 7.36347
10338019 5.43149
10338020 8.99238

Total number of rows: 241576

Table truncated, full table size 3999 Kbytes.




Supplementary file Size Download File type/resource
GSM754746_CB_14d_2.CEL.gz 4.0 Mb (ftp)(http) CEL
Processed data included within Sample table

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