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Sample GSM756533 Query DataSets for GSM756533
Status Public on May 03, 2012
Title 4F iNSC (late) rep1
Sample type RNA
 
Source name 4F iNSC (late) rep1
Organism Mus musculus
Characteristics nsc like: Yes
Extracted molecule total RNA
Extraction protocol RNA samples to be analysed on microarrays were prepared using QIAGEN RNeasy columns with on-column DNA digestion. 500 ng of total DNA-free RNA was used as input for labelled cRNA synthesis (Illumina TotalPrep RNA Amplification Kit - Ambion) following the manufacturer's instructions (IVT: 12h).
Label biotin
Label protocol Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays
 
Hybridization protocol Standard Illumina hybridization protocol
Scan protocol Standard Illumina scanning protocol
Description replicate 1
Data processing The bead intensities were mapped to gene information using BeadStudio 3.2 (Illumina), background correction was perform using the Affymetrix Robust Multi-array Analysis (RMA) background correction model, variance stabilization was performed using the log2 scaling and gene expression normalization was calculated with the quantile method implemented in the lumi package of R-Bioconductor.
 
Submission date Jul 07, 2011
Last update date Jan 15, 2022
Contact name Marcos J. Araúzo-Bravo
E-mail(s) mararabra@yahoo.co.uk
Phone +34 943 00 6108
Organization name Max Planck Institute for Molecular Biomedicine
Department Cell and Developmental Biology
Lab Computational Biology and Bionformatics
Street address Rogentstrasse
City Muenster
ZIP/Postal code 48149
Country Germany
 
Platform ID GPL6885
Series (1)
GSE30500 Direct Reprogramming of Fibroblasts into Neural Stem Cells by Defined Factors

Data table header descriptions
ID_REF
VALUE quantile normalized signal intensity

Data table
ID_REF VALUE
ILMN_1212607 3.727499
ILMN_1212612 2.090061
ILMN_1212619 5.715474
ILMN_1212628 2.076764
ILMN_1212632 4.091475
ILMN_1212636 11.984923
ILMN_1212637 9.484471
ILMN_1212645 3.650111
ILMN_1212648 7.039530
ILMN_1212653 8.237390
ILMN_1212672 8.106024
ILMN_1212682 4.473829
ILMN_1212683 2.033904
ILMN_1212685 2.274225
ILMN_1212692 2.385415
ILMN_1212693 5.237297
ILMN_1212695 2.399449
ILMN_1212698 2.282948
ILMN_1212702 1.798616
ILMN_1212703 3.448498

Total number of rows: 25697

Table truncated, full table size 554 Kbytes.




Supplementary data files not provided
Processed data included within Sample table

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