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Sample GSM75830 Query DataSets for GSM75830
Status Public on Dec 22, 2005
Title BJ5459 spt10-C388S mutant. Data set #1
Sample type RNA
 
Source name Yeast cells in log phase of growth in synthetic medium.
Organism Saccharomyces cerevisiae
Characteristics Yeast strain based on BJ5459 carrying the spt10-C388S mutation (and a C-terminal 3HA tag). BJ-SPT10(C388S)-HA cells were grown in synthetic complete medium to A600 = 0.6. RNA was prepared using an RNeasy kit (Qiagen). This is #1 of three independently prepared samples.
Extracted molecule total RNA
Label Bioytinylated cRNAs detected by fluorescently-labelled streptavidin.
 
Description No other details.
Data processing Each VALUE was calculated using Affymetrix GCOS software.
 
Submission date Sep 22, 2005
Last update date Sep 23, 2005
Contact name David Johannes Clark
E-mail(s) clarkda@mail.nih.gov
Phone 3014966966
Organization name NICHD, NIH
Department DDB
Lab SCGE
Street address 6 Center Drive Bldg 6A Rm 2A02
City Bethesda
State/province MD
ZIP/Postal code 20892
Country USA
 
Platform ID GPL90
Series (1)
GSE3354 Global effects of spt10 mutations on gene expression in budding yeast.

Data table header descriptions
ID_REF
Stat pairs The number of probe pairs that are associated with a given probe set.
Stat pairs used The number of probe pairs that were used as a basis for calculation of Value. For example, this number would reflect any probe pairs that were masked.
VALUE An overall estimate of transcript abundance for the particular sample. Signal values are roughly proportional to the amount of transcript present.
ABS_CALL A"call" of the presence or absence of expression of a transcript. This call indicates whether one can distinguish the signal against the background variability. P = present; M = marginal (ambiguous); A = absent.
DETECTION P-VALUE An indicator of the statistical significance of the "present" detection call. The lower the value, the greater the significance.

Data table
ID_REF Stat pairs Stat pairs used VALUE ABS_CALL DETECTION P-VALUE
AFFX-MurIL2_at 20 20 3.5 A 0.993813
AFFX-MurIL10_at 20 20 1.4 A 0.993129
AFFX-MurIL4_at 20 20 1.3 A 0.916427
AFFX-MurFAS_at 20 20 10.1 A 0.834139
AFFX-BioB-5_at 20 20 174.1 P 0.002275
AFFX-BioB-M_at 20 20 293.5 P 0.000095
AFFX-BioB-3_at 20 20 242.1 P 0.000147
AFFX-BioC-5_at 20 20 573.9 P 0.000110
AFFX-BioC-3_at 20 20 825.8 P 0.000044
AFFX-BioDn-5_at 20 20 899.6 P 0.000044
AFFX-BioDn-3_at 20 20 5238.0 P 0.000044
AFFX-CreX-5_at 20 20 6179.2 P 0.000044
AFFX-CreX-3_at 20 20 7784.6 P 0.000044
AFFX-BioB-5_st 20 20 6.1 A 0.631562
AFFX-BioB-M_st 20 20 5.0 A 0.843269
AFFX-BioB-3_st 20 20 1.7 A 0.957038
AFFX-BioC-5_st 20 20 2.6 A 0.941556
AFFX-BioC-3_st 20 20 0.8 A 0.937071
AFFX-BioDn-5_st 20 20 7.8 A 0.514890
AFFX-BioDn-3_st 20 20 35.9 A 0.089478

Total number of rows: 9335

Table truncated, full table size 283 Kbytes.




Supplementary data files not provided

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