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Sample GSM758309 Query DataSets for GSM758309
Status Public on Jul 18, 2011
Title intestine, BlimpWT, rep3
Sample type RNA
 
Source name Mid-intestine, WT
Organism Mus musculus
Characteristics strain: C57BL/6
genotype: Blimp wild type
tissue: mid-intestine
age: postnatal day 7
Treatment protocol Conditional knockout of Prdm1 (Blimp1) crossed into the VillinCre allele.
Growth protocol Specific pathogen-free (SPF) caging.
Extracted molecule total RNA
Extraction protocol Trizol, followed by Qiagen RNeasy cleanup.
Label Biotin
Label protocol Ambion cRNA synthesis for illumina.
 
Hybridization protocol According to illumina instructions.
Scan protocol According to illumina instructions on a BeadArray 500GX scanner.
Description 5388876105_E.AVG_Signal
VillinCre-Blimpwt
Data processing All transcripts were taken from data with standard background subtraction in illumina Beadstudio, and all genes that had low intensity values (<5) were omitted. Subsequently, quantile normalization was performed.
 
Submission date Jul 11, 2011
Last update date Jul 18, 2011
Contact name Jarom Heijmans
E-mail(s) j.heijmans@amc.nl
Organization name Academic Medical Center
Department Tytgat Institute for liver and intestinal research
Lab van den Brink Group
Street address Meibergdreef 69-71
City Amsterdam
ZIP/Postal code 1105 BK
Country Netherlands
 
Platform ID GPL6885
Series (1)
GSE30556 VillinCre-Blimpflox mice postpartum day 7

Data table header descriptions
ID_REF
VALUE Normalized signal intensity
Detection Pval

Data table
ID_REF VALUE Detection Pval
ILMN_1212607 22.1 0.04887
ILMN_1212612 3.7 0.30952
ILMN_1212619 -3.1 0.53383
ILMN_1212628 8.6 0.19549
ILMN_1212632 42.9 0.00627
ILMN_1212636 1317.3 0
ILMN_1212637 1037.4 0
ILMN_1212645 10.7 0.16165
ILMN_1212648 175.7 0
ILMN_1212653 90.8 0
ILMN_1212672 85.3 0
ILMN_1212682 25.8 0.03008
ILMN_1212683 -8.7 0.7381
ILMN_1212685 -13.5 0.89474
ILMN_1212692 1559.3 0
ILMN_1212693 40.2 0.00752
ILMN_1212695 5.3 0.26692
ILMN_1212698 -13.6 0.89599
ILMN_1212702 24143.6 0
ILMN_1212703 65.1 0.00125

Total number of rows: 25697

Table truncated, full table size 599 Kbytes.




Supplementary data files not provided
Raw data are available on Series record
Processed data included within Sample table

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