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Sample GSM758310 Query DataSets for GSM758310
Status Public on Jul 18, 2011
Title intestine, BlimpWT, rep4
Sample type RNA
 
Source name Mid-intestine, WT
Organism Mus musculus
Characteristics strain: C57BL/6
genotype: Blimp wild type
tissue: mid-intestine
age: postnatal day 7
Treatment protocol Conditional knockout of Prdm1 (Blimp1) crossed into the VillinCre allele.
Growth protocol Specific pathogen-free (SPF) caging.
Extracted molecule total RNA
Extraction protocol Trizol, followed by Qiagen RNeasy cleanup.
Label Biotin
Label protocol Ambion cRNA synthesis for illumina.
 
Hybridization protocol According to illumina instructions.
Scan protocol According to illumina instructions on a BeadArray 500GX scanner.
Description 5388876105_F.AVG_Signal
VillinCre-Blimpwt
Data processing All transcripts were taken from data with standard background subtraction in illumina Beadstudio, and all genes that had low intensity values (<5) were omitted. Subsequently, quantile normalization was performed.
 
Submission date Jul 11, 2011
Last update date Jul 18, 2011
Contact name Jarom Heijmans
E-mail(s) j.heijmans@amc.nl
Organization name Academic Medical Center
Department Tytgat Institute for liver and intestinal research
Lab van den Brink Group
Street address Meibergdreef 69-71
City Amsterdam
ZIP/Postal code 1105 BK
Country Netherlands
 
Platform ID GPL6885
Series (1)
GSE30556 VillinCre-Blimpflox mice postpartum day 7

Data table header descriptions
ID_REF
VALUE Normalized signal intensity
Detection Pval

Data table
ID_REF VALUE Detection Pval
ILMN_1212607 39.1 0.01128
ILMN_1212612 12.1 0.16416
ILMN_1212619 -6.9 0.68546
ILMN_1212628 -5.1 0.62406
ILMN_1212632 38.5 0.01253
ILMN_1212636 1071.8 0
ILMN_1212637 910.1 0
ILMN_1212645 7.4 0.24311
ILMN_1212648 219.9 0
ILMN_1212653 83.1 0.00125
ILMN_1212672 79.8 0.00125
ILMN_1212682 0.2 0.42231
ILMN_1212683 -9 0.76065
ILMN_1212685 4 0.32832
ILMN_1212692 2103.7 0
ILMN_1212693 38.1 0.01253
ILMN_1212695 -9.1 0.7619
ILMN_1212698 -12.7 0.8584
ILMN_1212702 32727.8 0
ILMN_1212703 86.8 0.00125

Total number of rows: 25697

Table truncated, full table size 604 Kbytes.




Supplementary data files not provided
Raw data are available on Series record
Processed data included within Sample table

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