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Sample GSM758311 Query DataSets for GSM758311
Status Public on Jul 18, 2011
Title intestine, BlimpWT, rep5
Sample type RNA
 
Source name Mid-intestine, WT
Organism Mus musculus
Characteristics strain: C57BL/6
genotype: Blimp wild type
tissue: mid-intestine
age: postnatal day 7
Treatment protocol Conditional knockout of Prdm1 (Blimp1) crossed into the VillinCre allele.
Growth protocol Specific pathogen-free (SPF) caging.
Extracted molecule total RNA
Extraction protocol Trizol, followed by Qiagen RNeasy cleanup.
Label Biotin
Label protocol Ambion cRNA synthesis for illumina.
 
Hybridization protocol According to illumina instructions.
Scan protocol According to illumina instructions on a BeadArray 500GX scanner.
Description 5388876090_B.AVG_Signal
VillinCre-Blimpwt
Data processing All transcripts were taken from data with standard background subtraction in illumina Beadstudio, and all genes that had low intensity values (<5) were omitted. Subsequently, quantile normalization was performed.
 
Submission date Jul 11, 2011
Last update date Jul 18, 2011
Contact name Jarom Heijmans
E-mail(s) j.heijmans@amc.nl
Organization name Academic Medical Center
Department Tytgat Institute for liver and intestinal research
Lab van den Brink Group
Street address Meibergdreef 69-71
City Amsterdam
ZIP/Postal code 1105 BK
Country Netherlands
 
Platform ID GPL6885
Series (1)
GSE30556 VillinCre-Blimpflox mice postpartum day 7

Data table header descriptions
ID_REF
VALUE Normalized signal intensity
Detection Pval

Data table
ID_REF VALUE Detection Pval
ILMN_1212607 25 0.05013
ILMN_1212612 1.5 0.37845
ILMN_1212619 5.9 0.28446
ILMN_1212628 -10.5 0.78446
ILMN_1212632 28.4 0.03885
ILMN_1212636 1209.3 0
ILMN_1212637 886.4 0
ILMN_1212645 -2 0.48872
ILMN_1212648 271.6 0
ILMN_1212653 48.5 0.00627
ILMN_1212672 71.7 0.00125
ILMN_1212682 11.9 0.16917
ILMN_1212683 2.1 0.36341
ILMN_1212685 -6.8 0.68045
ILMN_1212692 2280.8 0
ILMN_1212693 44 0.00627
ILMN_1212695 -7.1 0.68672
ILMN_1212698 -14.3 0.88221
ILMN_1212702 24530.3 0
ILMN_1212703 71.1 0.00125

Total number of rows: 25697

Table truncated, full table size 602 Kbytes.




Supplementary data files not provided
Raw data are available on Series record
Processed data included within Sample table

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