NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Sample GSM758316 Query DataSets for GSM758316
Status Public on Jul 18, 2011
Title intestine, BlimpKO, rep2
Sample type RNA
 
Source name Mid-intestine, KO
Organism Mus musculus
Characteristics strain: C57BL/6
genotype: Blimp knockout
tissue: mid-intestine
age: postnatal day 7
Treatment protocol Conditional knockout of Prdm1 (Blimp1) crossed into the VillinCre allele.
Growth protocol Specific pathogen-free (SPF) caging.
Extracted molecule total RNA
Extraction protocol Trizol, followed by Qiagen RNeasy cleanup.
Label Biotin
Label protocol Ambion cRNA synthesis for illumina.
 
Hybridization protocol According to illumina instructions.
Scan protocol According to illumina instructions on a BeadArray 500GX scanner.
Description 5388876105_C.AVG_Signal
VillinCre-Blimpflox
Data processing All transcripts were taken from data with standard background subtraction in illumina Beadstudio, and all genes that had low intensity values (<5) were omitted. Subsequently, quantile normalization was performed.
 
Submission date Jul 11, 2011
Last update date Jul 18, 2011
Contact name Jarom Heijmans
E-mail(s) j.heijmans@amc.nl
Organization name Academic Medical Center
Department Tytgat Institute for liver and intestinal research
Lab van den Brink Group
Street address Meibergdreef 69-71
City Amsterdam
ZIP/Postal code 1105 BK
Country Netherlands
 
Platform ID GPL6885
Series (1)
GSE30556 VillinCre-Blimpflox mice postpartum day 7

Data table header descriptions
ID_REF
VALUE Normalized signal intensity
Detection Pval

Data table
ID_REF VALUE Detection Pval
ILMN_1212607 20 0.08271
ILMN_1212612 6.3 0.26441
ILMN_1212619 2.6 0.36341
ILMN_1212628 7.8 0.2406
ILMN_1212632 12.9 0.16416
ILMN_1212636 1092 0
ILMN_1212637 1267.3 0
ILMN_1212645 10.1 0.20175
ILMN_1212648 168.5 0
ILMN_1212653 43.8 0.00877
ILMN_1212672 105 0
ILMN_1212682 12.1 0.17419
ILMN_1212683 -20 0.97744
ILMN_1212685 -13 0.86842
ILMN_1212692 2292.9 0
ILMN_1212693 64.1 0.00251
ILMN_1212695 -5.8 0.63659
ILMN_1212698 -11.7 0.83459
ILMN_1212702 15134.7 0
ILMN_1212703 77.9 0

Total number of rows: 25697

Table truncated, full table size 599 Kbytes.




Supplementary data files not provided
Raw data are available on Series record
Processed data included within Sample table

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap