|
Status |
Public on Jul 18, 2011 |
Title |
intestine, BlimpKO, rep3 |
Sample type |
RNA |
|
|
Source name |
Mid-intestine, KO
|
Organism |
Mus musculus |
Characteristics |
strain: C57BL/6 genotype: Blimp knockout tissue: mid-intestine age: postnatal day 7
|
Treatment protocol |
Conditional knockout of Prdm1 (Blimp1) crossed into the VillinCre allele.
|
Growth protocol |
Specific pathogen-free (SPF) caging.
|
Extracted molecule |
total RNA |
Extraction protocol |
Trizol, followed by Qiagen RNeasy cleanup.
|
Label |
Biotin
|
Label protocol |
Ambion cRNA synthesis for illumina.
|
|
|
Hybridization protocol |
According to illumina instructions.
|
Scan protocol |
According to illumina instructions on a BeadArray 500GX scanner.
|
Description |
5388876105_G.AVG_Signal VillinCre-Blimpflox
|
Data processing |
All transcripts were taken from data with standard background subtraction in illumina Beadstudio, and all genes that had low intensity values (<5) were omitted. Subsequently, quantile normalization was performed.
|
|
|
Submission date |
Jul 11, 2011 |
Last update date |
Jul 18, 2011 |
Contact name |
Jarom Heijmans |
E-mail(s) |
j.heijmans@amc.nl
|
Organization name |
Academic Medical Center
|
Department |
Tytgat Institute for liver and intestinal research
|
Lab |
van den Brink Group
|
Street address |
Meibergdreef 69-71
|
City |
Amsterdam |
ZIP/Postal code |
1105 BK |
Country |
Netherlands |
|
|
Platform ID |
GPL6885 |
Series (1) |
GSE30556 |
VillinCre-Blimpflox mice postpartum day 7 |
|