|
| Status |
Public on Jul 18, 2011 |
| Title |
intestine, BlimpKO, rep6 |
| Sample type |
RNA |
| |
|
| Source name |
Mid-intestine, KO
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57BL/6
genotype: Blimp knockout
tissue: mid-intestine
age: postnatal day 7
|
| Treatment protocol |
Conditional knockout of Prdm1 (Blimp1) crossed into the VillinCre allele.
|
| Growth protocol |
Specific pathogen-free (SPF) caging.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol, followed by Qiagen RNeasy cleanup.
|
| Label |
Biotin
|
| Label protocol |
Ambion cRNA synthesis for illumina.
|
| |
|
| Hybridization protocol |
According to illumina instructions.
|
| Scan protocol |
According to illumina instructions on a BeadArray 500GX scanner.
|
| Description |
5388876090_C.AVG_Signal
VillinCre-Blimpflox
|
| Data processing |
All transcripts were taken from data with standard background subtraction in illumina Beadstudio, and all genes that had low intensity values (<5) were omitted. Subsequently, quantile normalization was performed.
|
| |
|
| Submission date |
Jul 11, 2011 |
| Last update date |
Jul 18, 2011 |
| Contact name |
Jarom Heijmans |
| E-mail(s) |
j.heijmans@amc.nl
|
| Organization name |
Academic Medical Center
|
| Department |
Tytgat Institute for liver and intestinal research
|
| Lab |
van den Brink Group
|
| Street address |
Meibergdreef 69-71
|
| City |
Amsterdam |
| ZIP/Postal code |
1105 BK |
| Country |
Netherlands |
| |
|
| Platform ID |
GPL6885 |
| Series (1) |
| GSE30556 |
VillinCre-Blimpflox mice postpartum day 7 |
|
